TY - JOUR
T1 - Mimicking the topography of the epidermal-dermal interface with elastomer substrates
AU - Viswanathan, Priyalakshmi
AU - Guvendiren, Murat
AU - Chua, Wesley
AU - Telerman, Stephanie B.
AU - Liakath-Ali, Kifayathullah
AU - Burdick, Jason A.
AU - Watt, Fiona M.
N1 - Funding Information:
The authors gratefully acknowledge the expert technical support of the Nikon Imaging Centre at King''s College London and use of Core Facilities provided by the financial support from the Department of Health via the National Institute for Health Research (NIHR) Comprehensive Biomedical Research Centre award to Guy''s & St Thomas''s NHS Foundation Trust in partnership with King''s College London and King''s College Hospital NHS Foundation Trust. Adult patient samples were provided by King''s Health Partners Cancer Biobank, London, UK, which is supported by the Experimental Cancer Medicine Centre at King''s College London and the Department of Health via the NIHR Comprehensive Biomedical Research Centre award.
Publisher Copyright:
© 2016 The Royal Society of Chemistry.
PY - 2016/1
Y1 - 2016/1
N2 - In human skin the interface between the epidermis and dermis is not flat, but undulates. The dimensions of the undulations change as a function of age and disease. Epidermal stem cell clusters lie in specific locations relative to the undulations; however, whether their location affects their properties is unknown. To explore this, we developed a two-step protocol to create patterned substrates that mimic the topographical features of the human epidermal-dermal interface. Substrates with negative patterns were first fabricated by exposing a photocurable formulation to light, controlling the topographical features (such as diameter, height and center-to-center distance) by the photomask pattern dimensions and UV crosslinking time. The negative pattern was then translated to PDMS elastomer to fabricate substrates with 8 unique surface topographies on which primary human keratinocytes were cultured. We found that cells were patterned according to topography, and that separate cues determined the locations of stem cells, differentiated cells and proliferating cells. The biomimetic platform we have developed will be useful for probing the effect of topography on stem cell behaviour.
AB - In human skin the interface between the epidermis and dermis is not flat, but undulates. The dimensions of the undulations change as a function of age and disease. Epidermal stem cell clusters lie in specific locations relative to the undulations; however, whether their location affects their properties is unknown. To explore this, we developed a two-step protocol to create patterned substrates that mimic the topographical features of the human epidermal-dermal interface. Substrates with negative patterns were first fabricated by exposing a photocurable formulation to light, controlling the topographical features (such as diameter, height and center-to-center distance) by the photomask pattern dimensions and UV crosslinking time. The negative pattern was then translated to PDMS elastomer to fabricate substrates with 8 unique surface topographies on which primary human keratinocytes were cultured. We found that cells were patterned according to topography, and that separate cues determined the locations of stem cells, differentiated cells and proliferating cells. The biomimetic platform we have developed will be useful for probing the effect of topography on stem cell behaviour.
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U2 - 10.1039/c5ib00238a
DO - 10.1039/c5ib00238a
M3 - Article
C2 - 26658424
AN - SCOPUS:84955482685
SN - 1757-9694
VL - 8
SP - 21
EP - 29
JO - Integrative Biology (United Kingdom)
JF - Integrative Biology (United Kingdom)
IS - 1
ER -