Multi-label Detection and Classification of Red Blood Cells in Microscopic Images

Wei Qiu, Jiaming Guo, Xiang Li, Mengjia Xu, Mo Zhang, Ning Guo, Quanzheng Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Cell detection and cell type classification from biomedical images play an important role for high-throughput imaging and various clinical application. While classification of single cell sample can be performed with standard computer vision and machine learning methods, analysis of multi-label samples (region containing congregating cells) is more challenging, as separation of individual cells can be difficult (e.g. touching cells) or even impossible (e.g. overlapping cells). As multi-instance images are common in analyzing Red Blood Cell (RBC) for Sickle Cell Disease (SCD) diagnosis, we develop and implement a multi-instance cell detection and classification framework to address this challenge. The framework firstly trains a region proposal model based on Region-based Convolutional Network (RCNN) to obtain bounding-boxes of regions potentially containing single or multiple cells from input microscopic images, which are extracted as image patches. High-level image features are then calculated from image patches through a pre-trained Convolutional Neural Network (CNN) with ResNet-50 structure. Using these image features inputs, six networks are then trained to make multi-label prediction of whether a given patch contains cells belonging to a specific cell type. As the six networks are trained with image patches consisting of both individual cells and touching/overlapping cells, they can effectively recognize cell types that are presented in multi-instance image samples. Finally, for the purpose of SCD testing, we train another machine learning classifier to predict whether the given image patch contains abnormal cell type based on outputs from the six networks. Testing result of the proposed framework shows that it can achieve good performance in automatic cell detection and classification.

Original languageEnglish (US)
Title of host publicationProceedings - 2020 IEEE International Conference on Big Data, Big Data 2020
EditorsXintao Wu, Chris Jermaine, Li Xiong, Xiaohua Tony Hu, Olivera Kotevska, Siyuan Lu, Weijia Xu, Srinivas Aluru, Chengxiang Zhai, Eyhab Al-Masri, Zhiyuan Chen, Jeff Saltz
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages4257-4263
Number of pages7
ISBN (Electronic)9781728162515
DOIs
StatePublished - Dec 10 2020
Externally publishedYes
Event8th IEEE International Conference on Big Data, Big Data 2020 - Virtual, Atlanta, United States
Duration: Dec 10 2020Dec 13 2020

Publication series

NameProceedings - 2020 IEEE International Conference on Big Data, Big Data 2020

Conference

Conference8th IEEE International Conference on Big Data, Big Data 2020
Country/TerritoryUnited States
CityVirtual, Atlanta
Period12/10/2012/13/20

All Science Journal Classification (ASJC) codes

  • Computer Networks and Communications
  • Information Systems
  • Information Systems and Management
  • Safety, Risk, Reliability and Quality

Keywords

  • cell classification
  • multi-instance object detection
  • sickle cell disease

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