Multi-material digital light processing bioprinting of hydrogel-based microfluidic chips

Anant Bhusal, Elvan Dogan, Hai Anh Nguyen, Olga Labutina, Daniel Nieto, Ali Khademhosseini, Amir K. Miri

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Recent advancements in digital-light-processing (DLP)-based bioprinting and hydrogel engineering have enabled novel developments in organs-on-chips. In this work, we designed and developed a multi-material, DLP-based bioprinter for rapid, one-step prototyping of hydrogel-based microfluidic chips. A composite hydrogel bioink based on poly-ethylene-glycol-diacrylate (PEGDA) and gelatin methacryloyl (GelMA) was optimized through varying the bioprinting parameters such as light exposure time, bioink composition, and layer thickness. We showed a wide range of mechanical properties of the microfluidic chips for various ratios of PEGDA:GelMA. Microfluidic features of hydrogel-based chips were then tested using dynamic flow experiments. Human-derived tumor cells were encapsulated in 3D bioprinted structures to demonstrate their bioactivity and cell-friendly environment. Cell seeding experiments then validated the efficacy of the selected bioinks for vascularized micro-tissues. Our biofabrication approach offers a useful tool for the rapid integration of micro-tissue models into organs-on-chips and high-throughput drug screening platforms.

Original languageEnglish (US)
Article number014103
JournalBiofabrication
Volume14
Issue number1
DOIs
StatePublished - Jan 2022
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Keywords

  • digital-light-processing
  • hydrogel models
  • microfluidics
  • organ-on-a-chip

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