Multiphoton angiogenesis and tumor biomarker imaging

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The imaging of tumors using techniques such as magnetic resonance imaging (MRI), single photon emission computed tomography (SPECT), positron emission tomography (PET), and near infrared fluorescence (NIRF) has seen significant advances in recent years. Two-photon fluorescence microscopy (2PFM) offers several advantages, such as deeper tissue penetration along with more confined excitation and emission that leads to an increase in imaging resolution. Although biomedical applications of two-photon fluorescence microscopy (2PFM) are steadily increasing, the technique still suffers from the lack of efficient, photostable two-photon absorbing fluorescent probes that possess high target specificity. In order to be truly useful for such applications, it is necessary to have not only an imaging component that undergoes two-photon absorption (2PA) at wavelengths longer than 700 nm, but also a vector that targets the fluorescent probe selectively to a particular tissue, cell, organelle, receptor, or protein. We describe approaches to 2PFM imaging that targets important biomarkers, including proteins that are expressed in tumors and the targeting of vasculature around the tumors rather than tumors themselves. In this work, we describe the preparation and the use of novel, efficient two-photon absorbing (2PA) fluorescent probes that target the vascular endothelial growth factor 2 (VEGFR-2), folate receptors, and integrin proteins. Comprehensive linear and nonlinear photophysical and biological characterization of the new probes will be presented along with 2PFM imaging.

Original languageEnglish (US)
Title of host publication2011 IEEE 61st Electronic Components and Technology Conference, ECTC 2011
Pages1314-1318
Number of pages5
DOIs
StatePublished - Jul 21 2011
Externally publishedYes
Event2011 61st Electronic Components and Technology Conference, ECTC 2011 - Lake Buena Vista, FL, United States
Duration: May 31 2011Jun 3 2011

Publication series

NameProceedings - Electronic Components and Technology Conference
ISSN (Print)0569-5503

Other

Other2011 61st Electronic Components and Technology Conference, ECTC 2011
CountryUnited States
CityLake Buena Vista, FL
Period5/31/116/3/11

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

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