N-type Ca2+ channels carry the largest current: Implications for nanodomains and transmitter release

Alexander M. Weber, Fiona K. Wong, Adele R. Tufford, Lyanne C. Schlichter, Victor Matveev, Elise F. Stanley

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Presynaptic terminals favor intermediate-conductance Ca V2.2 (N type) over high-conductance Ca V 1 (L type) channels for single-channel, Ca2+ nanodomain-triggered synaptic vesicle fusion. However, the standard Ca V 1>Ca V 2>Ca V 3 conductance hierarchy is based on recordings using nonphysiological divalent ion concentrations. We found that, with physiological Ca2+ gradients, the hierarchy was Ca V 2.2>Ca V 1>Ca V 3. Mathematical modeling predicts that the Ca V 2.2 Ca2+ nanodomain, which is ĝ̂1/425% more extensive than that generated by Ca V 1, can activate a calcium-fusion sensor located on the proximal face of the synaptic vesicle.

Original languageEnglish (US)
Pages (from-to)1348-1350
Number of pages3
JournalNature Neuroscience
Volume13
Issue number11
DOIs
StatePublished - Nov 2010

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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