Nanofibrous Snake Venom Hemostat

Vivek A. Kumar, Navindee C. Wickremasinghe, Siyu Shi, Jeffrey D. Hartgerink

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Controlling perioperative bleeding is of critical importance to minimize hemorrhaging and fatality. Patients on anticoagulant therapy such as heparin have diminished clotting potential and are at risk for hemorrhaging. Here we describe a self-assembling nanofibrous peptide hydrogel (termed SLac) that on its own can act as a physical barrier to blood loss. SLac was loaded with snake-venom derived Batroxobin (50 μg/mL) yielding a drug-loaded hydrogel (SB50). SB50 was potentiated to enhance clotting even in the presence of heparin. In vitro evaluation of fibrin and whole blood clotting helped identify appropriate concentrations for hemostasis in vivo. Batroxobin-loaded hydrogels rapidly (within 20s) stop bleeding in both normal and heparin-treated rats in a lateral liver incision model. Compared to standard of care, Gelfoam, and investigational hemostats such as Puramatrix, only SB50 showed rapid liver incision hemostasis post surgical application. This snake venom-loaded peptide hydrogel can be applied via syringe and conforms to the wound site resulting in hemostasis. This demonstrates a facile method for surgical hemostasis even in the presence of anticoagulant therapies.

Original languageEnglish (US)
Pages (from-to)1300-1305
Number of pages6
JournalACS Biomaterials Science and Engineering
Volume1
Issue number12
DOIs
StatePublished - Dec 14 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Keywords

  • hemostasis
  • multidomain peptide
  • self-assembly
  • supramolecular chemistry

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