Near-infrared photoactivatable control of Ca2+ signaling and optogenetic immunomodulation

Lian He, Yuanwei Zhang, Guolin Ma, Peng Tan, Zhanjun Li, Shengbing Zang, Xiang Wu, Ji Jing, Shaohai Fang, Lijuan Zhou, Youjun Wang, Yun Huang, Patrick G. Hogan, Gang Han, Yubin Zhou

Research output: Contribution to journalArticlepeer-review

131 Scopus citations

Abstract

The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed ’Opto-CRAC’) that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded ’photoactivatable adjuvant’ to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote and wireless control of Ca2+-modulated activities with tailored function.

Original languageEnglish (US)
Article numbere10024
JournaleLife
Volume4
Issue numberDECEMBER2015
DOIs
StatePublished - Dec 8 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'Near-infrared photoactivatable control of Ca<sup>2+</sup> signaling and optogenetic immunomodulation'. Together they form a unique fingerprint.

Cite this