Near-IR Two-Photon Fluorescent Sensor for K+ Imaging in Live Cells

Binglin Sui; Xiling Yue; Bosung Kim; Kevin D. Belfield

doi:10.1021/acsami.5b04506

Near-IR Two-Photon Fluorescent Sensor for K⁺ Imaging in Live Cells

Binglin Sui, Xiling Yue, Bosung Kim, Kevin D. Belfield

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A new two-photon excited fluorescent K⁺ sensor is reported. The sensor comprises three moieties, a highly selective K⁺ chelator as the K⁺ recognition unit, a boron-dipyrromethene (BODIPY) derivative modified with phenylethynyl groups as the fluorophore, and two polyethylene glycol chains to afford water solubility. The sensor displays very high selectivity (>52-fold) in detecting K⁺ over other physiological metal cations. Upon binding K⁺, the sensor switches from nonfluorescent to highly fluorescent, emitting red to near-IR (NIR) fluorescence. The sensor exhibited a good two-photon absorption cross section, 500 GM at 940 nm. Moreover, it is not sensitive to pH in the physiological pH range. Time-dependent cell imaging studies via both one- and two-photon fluorescence microscopy demonstrate that the sensor is suitable for dynamic K⁺ sensing in living cells.

Original language	English (US)
Pages (from-to)	17565-17568
Number of pages	4
Journal	ACS Applied Materials and Interfaces
Volume	7
Issue number	32
DOIs	https://doi.org/10.1021/acsami.5b04506
State	Published - Aug 19 2015

All Science Journal Classification (ASJC) codes

General Materials Science

Keywords

cell imaging
fluorescent sensors
potassium sensors
two-photon absorption
two-photon fluorescence microscopy

Access to Document

10.1021/acsami.5b04506

Cite this

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title = "Near-IR Two-Photon Fluorescent Sensor for K+ Imaging in Live Cells",

abstract = "A new two-photon excited fluorescent K+ sensor is reported. The sensor comprises three moieties, a highly selective K+ chelator as the K+ recognition unit, a boron-dipyrromethene (BODIPY) derivative modified with phenylethynyl groups as the fluorophore, and two polyethylene glycol chains to afford water solubility. The sensor displays very high selectivity (>52-fold) in detecting K+ over other physiological metal cations. Upon binding K+, the sensor switches from nonfluorescent to highly fluorescent, emitting red to near-IR (NIR) fluorescence. The sensor exhibited a good two-photon absorption cross section, 500 GM at 940 nm. Moreover, it is not sensitive to pH in the physiological pH range. Time-dependent cell imaging studies via both one- and two-photon fluorescence microscopy demonstrate that the sensor is suitable for dynamic K+ sensing in living cells.",

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author = "Binglin Sui and Xiling Yue and Bosung Kim and Belfield, {Kevin D.}",

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