Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

Maria B. Harreguy, Tracy S. Tran, Gal Haspel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Laser microsurgery allows the user to ablate cell bodies or disconnect nerve fibers by using a laser microbeam focused through a microscope. This technique was pioneered in C. elegans where it led to exciting discoveries in the fields of development and neurobiology. All neurons studied so far in C. elegans can regenerate and regrow axons and dendrites after injury, allowing studies of the molecular and cellular basis of neuroregeneration. In this chapter, we describe how to assemble and operate a platform for Yb-doped fiber laser microsurgery. The novel laser setup described here is a more robust, lower cost, and user-friendly alternative to other femtosecond-pulsed laser systems.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages319-328
Number of pages10
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2468
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Axotomy
  • Dendrotomy
  • Injury
  • Laser
  • Microscopy
  • Microsurgery
  • Regeneration

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