TY - GEN
T1 - New cross-linking quinoline- and quinolone-based luminescent lanthanide probes for sensitive labeling
AU - Pillai, Shyamala
AU - Wirpsza, Laura
AU - Kozlov, Maxim
AU - Marras, Salvatore A.E.
AU - Krasnoperov, Lev N.
AU - Mustaev, Arkady
PY - 2012
Y1 - 2012
N2 - New luminescent lanthanide chelates containing thiol-, amine-, and click-reactive groups in antenna-fluorophore moieties were synthesized. The chelates include diethylenetriaminepentaacetic acid (DTPA) coupled to two types of chromophores: 7-amino-4-trifluoromethyl-2(1H) quinolinone, and 7-amino-4-trifluoromethyl-2-alkoxyquinoline. The synthesized compounds were characterized using NMR, light absorption, steady-state and time-resolved fluorescent spectroscopy. Some of the compounds displayed high brightness with Tb3+, Eu3+, and Dy3+. Obtained reactive lanthanide chelates can be easily attached to biological molecules. The probes demonstrated high performance in molecular beaconbased DNA hybridization assays (sub-pico molar detection limit), in bacterial proteome labeling, and in live cell imaging.
AB - New luminescent lanthanide chelates containing thiol-, amine-, and click-reactive groups in antenna-fluorophore moieties were synthesized. The chelates include diethylenetriaminepentaacetic acid (DTPA) coupled to two types of chromophores: 7-amino-4-trifluoromethyl-2(1H) quinolinone, and 7-amino-4-trifluoromethyl-2-alkoxyquinoline. The synthesized compounds were characterized using NMR, light absorption, steady-state and time-resolved fluorescent spectroscopy. Some of the compounds displayed high brightness with Tb3+, Eu3+, and Dy3+. Obtained reactive lanthanide chelates can be easily attached to biological molecules. The probes demonstrated high performance in molecular beaconbased DNA hybridization assays (sub-pico molar detection limit), in bacterial proteome labeling, and in live cell imaging.
KW - Lanthanide ion probes
KW - click reaction
KW - molecular beacons
KW - time-resolved detection
UR - http://www.scopus.com/inward/record.url?scp=84859612320&partnerID=8YFLogxK
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U2 - 10.1117/12.921056
DO - 10.1117/12.921056
M3 - Conference contribution
AN - SCOPUS:84859612320
SN - 9780819488763
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
T2 - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
Y2 - 23 January 2012 through 25 January 2012
ER -