New cross-linking quinoline- and quinolone-based luminescent lanthanide probes for sensitive labeling

Shyamala Pillai, Laura Wirpsza, Maxim Kozlov, Salvatore A.E. Marras, Lev Krasnoperov, Arkady Mustaev

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

New luminescent lanthanide chelates containing thiol-, amine-, and click-reactive groups in antenna-fluorophore moieties were synthesized. The chelates include diethylenetriaminepentaacetic acid (DTPA) coupled to two types of chromophores: 7-amino-4-trifluoromethyl-2(1H) quinolinone, and 7-amino-4-trifluoromethyl-2-alkoxyquinoline. The synthesized compounds were characterized using NMR, light absorption, steady-state and time-resolved fluorescent spectroscopy. Some of the compounds displayed high brightness with Tb3+, Eu3+, and Dy3+. Obtained reactive lanthanide chelates can be easily attached to biological molecules. The probes demonstrated high performance in molecular beaconbased DNA hybridization assays (sub-pico molar detection limit), in bacterial proteome labeling, and in live cell imaging.

Original languageEnglish (US)
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
DOIs
StatePublished - Apr 16 2012
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV - San Francisco, CA, United States
Duration: Jan 23 2012Jan 25 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8233
ISSN (Print)1605-7422

Other

OtherReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
CountryUnited States
CitySan Francisco, CA
Period1/23/121/25/12

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Keywords

  • Lanthanide ion probes
  • click reaction
  • molecular beacons
  • time-resolved detection

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