Novel hollow fiber immobilization techniques for whole cells and advanced bioreactors

Rajesh Shukla, Whankoo Kang, K. K. Sirkar

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


A novel microporous hollow fiber membrane-based immobilization technique for whole cells has been developed. Yeast cells (Saccharomyces cerevisiae) were grown on chopped hydrophobic microporous hollow fibers as well as on hydrophilic hollow fibers. This immobilization support was used to carry out fermentation in a tubular bioreactor. Air was passed from time to time to facilitate cell growth. The microbial culture reached a very high cell density level of around 1010/mL of fiber lumen volume. An ethanol concentration of 45 g/L and productivity of 41 g/L-h were obtained with an initial glucose concentration of 100 g/L. The present technique does not have the shortcomings of conventional immobilization methods.

Original languageEnglish (US)
Pages (from-to)571-586
Number of pages16
JournalApplied Biochemistry and Biotechnology
Issue number1
StatePublished - Jan 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology


  • Immobilization
  • fermentation
  • hollow-fibers
  • microporous
  • yeast


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