Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

Dao M. Nguyen, Xuhua Wang, Hyo Yang Ahn, Luis Rodriguez, Mikhailo V. Bondar, Kevin D. Belfield

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn2+, resulting in a large binding constant (1 × 109) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn2+. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2+ into HeLa cells and reversible Zn 2+ binding, demonstrating the potential of this probe for zinc ion sensing.

Original languageEnglish (US)
Pages (from-to)2978-2981
Number of pages4
JournalACS Applied Materials and Interfaces
Issue number11
StatePublished - Nov 24 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Materials Science


  • 1,2,3-triazolyl-fluorene
  • binding constant
  • fluorescent probe
  • metal sensing
  • two-photon microscopy bioimaging
  • zinc ion sensing


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