Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

Dao M. Nguyen; Xuhua Wang; Hyo Yang Ahn; Luis Rodriguez; Mikhailo V. Bondar; Kevin D. Belfield

doi:10.1021/am100719v

Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

Dao M. Nguyen, Xuhua Wang, Hyo Yang Ahn, Luis Rodriguez, Mikhailo V. Bondar, Kevin D. Belfield

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn²⁺, resulting in a large binding constant (1 × 10⁹) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn²⁺. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn²⁺ into HeLa cells and reversible Zn ²⁺ binding, demonstrating the potential of this probe for zinc ion sensing.

Original language	English (US)
Pages (from-to)	2978-2981
Number of pages	4
Journal	ACS Applied Materials and Interfaces
Volume	2
Issue number	11
DOIs	https://doi.org/10.1021/am100719v
State	Published - Nov 24 2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Materials Science

Keywords

1,2,3-triazolyl-fluorene
binding constant
fluorescent probe
metal sensing
two-photon microscopy bioimaging
zinc ion sensing

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10.1021/am100719v

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title = "Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing",

abstract = "A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn2+, resulting in a large binding constant (1 × 109) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn2+. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2+ into HeLa cells and reversible Zn 2+ binding, demonstrating the potential of this probe for zinc ion sensing.",

keywords = "1,2,3-triazolyl-fluorene, binding constant, fluorescent probe, metal sensing, two-photon microscopy bioimaging, zinc ion sensing",

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T1 - Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

AU - Nguyen, Dao M.

AU - Wang, Xuhua

AU - Ahn, Hyo Yang

AU - Rodriguez, Luis

AU - Bondar, Mikhailo V.

AU - Belfield, Kevin D.

PY - 2010/11/24

Y1 - 2010/11/24

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AB - A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn2+, resulting in a large binding constant (1 × 109) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn2+. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2+ into HeLa cells and reversible Zn 2+ binding, demonstrating the potential of this probe for zinc ion sensing.

KW - 1,2,3-triazolyl-fluorene

KW - binding constant

KW - fluorescent probe

KW - metal sensing

KW - two-photon microscopy bioimaging

KW - zinc ion sensing

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ER -

Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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