A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn2+, resulting in a large binding constant (1 × 109) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn2+. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2+ into HeLa cells and reversible Zn 2+ binding, demonstrating the potential of this probe for zinc ion sensing.
All Science Journal Classification (ASJC) codes
- General Materials Science
- binding constant
- fluorescent probe
- metal sensing
- two-photon microscopy bioimaging
- zinc ion sensing