Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

Dao M. Nguyen; Xuhua Wang; Hyo Yang Ahn; Luis Rodriguez; Mikhailo V. Bondar; Kevin D. Belfield

doi:10.1021/am100719v

Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

Dao M. Nguyen, Xuhua Wang, Hyo Yang Ahn, Luis Rodriguez, Mikhailo V. Bondar, Kevin D. Belfield

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn²⁺, resulting in a large binding constant (1 × 10⁹) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn²⁺. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn²⁺ into HeLa cells and reversible Zn ²⁺ binding, demonstrating the potential of this probe for zinc ion sensing.

Original language	English (US)
Pages (from-to)	2978-2981
Number of pages	4
Journal	ACS Applied Materials and Interfaces
Volume	2
Issue number	11
DOIs	https://doi.org/10.1021/am100719v
State	Published - Nov 24 2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Materials Science

Keywords

1,2,3-triazolyl-fluorene
binding constant
fluorescent probe
metal sensing
two-photon microscopy bioimaging
zinc ion sensing

Access to Document

10.1021/am100719v

Cite this

@article{1e0849ebf87b4821a326ec3d25f30341,

title = "Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing",

abstract = "A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn2+, resulting in a large binding constant (1 × 109) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn2+. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2+ into HeLa cells and reversible Zn 2+ binding, demonstrating the potential of this probe for zinc ion sensing.",

keywords = "1,2,3-triazolyl-fluorene, binding constant, fluorescent probe, metal sensing, two-photon microscopy bioimaging, zinc ion sensing",

author = "Nguyen, \{Dao M.\} and Xuhua Wang and Ahn, \{Hyo Yang\} and Luis Rodriguez and Bondar, \{Mikhailo V.\} and Belfield, \{Kevin D.\}",

year = "2010",

month = nov,

day = "24",

doi = "10.1021/am100719v",

language = "English (US)",

volume = "2",

pages = "2978--2981",

journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

number = "11",

}

TY - JOUR

T1 - Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

AU - Nguyen, Dao M.

AU - Wang, Xuhua

AU - Ahn, Hyo Yang

AU - Rodriguez, Luis

AU - Bondar, Mikhailo V.

AU - Belfield, Kevin D.

PY - 2010/11/24

Y1 - 2010/11/24

N2 - A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn2+, resulting in a large binding constant (1 × 109) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn2+. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2+ into HeLa cells and reversible Zn 2+ binding, demonstrating the potential of this probe for zinc ion sensing.

AB - A hydrophilic bis(1,2,3-triazolyl)fluorene derivative was synthesized as a multi-photon-absorbing, zinc-ion-sensing fluorescent probe. The fluorescence response was approximately five-fold greater in presence of Zn2+, resulting in a large binding constant (1 × 109) for a 1:2 ligand to zinc complex. A four-fold increase in the two-photon absorption cross section was achieved upon binding Zn2+. In vitro two-photon fluorescence microscopy imaging revealed a significant fluorescence increase upon introduction of Zn2+ into HeLa cells and reversible Zn 2+ binding, demonstrating the potential of this probe for zinc ion sensing.

KW - 1,2,3-triazolyl-fluorene

KW - binding constant

KW - fluorescent probe

KW - metal sensing

KW - two-photon microscopy bioimaging

KW - zinc ion sensing

UR - https://www.scopus.com/pages/publications/79151481873

UR - https://www.scopus.com/inward/citedby.url?scp=79151481873&partnerID=8YFLogxK

U2 - 10.1021/am100719v

DO - 10.1021/am100719v

M3 - Article

C2 - 21028841

AN - SCOPUS:79151481873

SN - 1944-8244

VL - 2

SP - 2978

EP - 2981

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 11

ER -

Novel hydrophilic bis(1,2,3-triazolyl)fluorenyl probe for in vitro zinc ion sensing

Abstract

All Science Journal Classification (ASJC) codes

Keywords

Access to Document

Other files and links

Fingerprint

Cite this