Optically computed phase microscopy for quantitative dynamic imaging of label-free cells and nanoparticles

Xuan Liu, Zhaoxiong Wan, Yuanwei Zhang, Yuwei Liu

Research output: Contribution to journalArticlepeer-review

Abstract

Numerous drug delivery systems based on nanoparticles have been developed, such as those used in BioNTech/Pfizer’s and Moderna’s Covid vaccines. Knowledge on mechanical interactions between cells and nanoparticles is critical to advance the efficiency and safety of these drug delivery systems. To quantitatively track the motion of cell (transparent) and nanoparticles (nontransparent) with nanometer displacement sensitivity, we investigate a novel imaging technology, optically computed phase microscopy (OCPM) that processes 3D spatial-spectral data through optical computation. We demonstrate that OCPM has the capability to image the motion of cells and magnetic nanoparticles that are mechanically excited by an external magnetic field, quantitatively and in the en face plane.

Original languageEnglish (US)
Pages (from-to)514-524
Number of pages11
JournalBiomedical Optics Express
Volume13
Issue number1
DOIs
StatePublished - 2021

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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