Optimum nitrogen concentration supporting maximum crude oil biodegradation in microcosms

Xiaoming Du; Paul Reeser; Makram T. Suidan; Tiehong Huang; Moustafa Moteleb; Michael C. Boufadel; Albert D. Venosa

Optimum nitrogen concentration supporting maximum crude oil biodegradation in microcosms

Xiaoming Du, Paul Reeser, Makram T. Suidan, Tiehong Huang, Moustafa Moteleb, Michael C. Boufadel, Albert D. Venosa

Research output: Contribution to conference › Paper › peer-review

Abstract

The purpose of this study was to determine the minimum nitrogen concentration needed by microorganisms to biodegrade crude oil hydrocarbons attached to sand particles in a microcosm simulating a low energy marine beach. The experimental approach involved mixing clean sand with weathered Alaska North Slope crude oil and monitoring biodegradation in continuous-flow beach microcosms over a 40-day time period. Two experiments were conducted with different nitrate-N concentrations in the feed (0, 0.25, 0.5, 1.0, 2.5, 5.0 mg/L in the first experiment and 2.5, 5.0, 10, 25, and 50 mg/L in the second). Experimental results indicated that nitrate concentrations below approximately 10 mg/L limited the rate of crude oil biodegradation. This conclusion was supported by oxygen uptake, CO ₂ production, nitrate consumption, and biomass growth data.

Original language	English (US)
Pages	5979-5984
Number of pages	6
State	Published - 2005
Externally published	Yes
Event	2005 International Oil Spill Conference, IOSC 2005 - Miami Beach, FL, United States Duration: May 15 2005 → May 19 2005

Other

Other	2005 International Oil Spill Conference, IOSC 2005
Country/Territory	United States
City	Miami Beach, FL
Period	5/15/05 → 5/19/05

All Science Journal Classification (ASJC) codes

General Engineering

Cite this

@conference{13c3718a333446b68574ed969835948f,

title = "Optimum nitrogen concentration supporting maximum crude oil biodegradation in microcosms",

abstract = "The purpose of this study was to determine the minimum nitrogen concentration needed by microorganisms to biodegrade crude oil hydrocarbons attached to sand particles in a microcosm simulating a low energy marine beach. The experimental approach involved mixing clean sand with weathered Alaska North Slope crude oil and monitoring biodegradation in continuous-flow beach microcosms over a 40-day time period. Two experiments were conducted with different nitrate-N concentrations in the feed (0, 0.25, 0.5, 1.0, 2.5, 5.0 mg/L in the first experiment and 2.5, 5.0, 10, 25, and 50 mg/L in the second). Experimental results indicated that nitrate concentrations below approximately 10 mg/L limited the rate of crude oil biodegradation. This conclusion was supported by oxygen uptake, CO 2 production, nitrate consumption, and biomass growth data.",

author = "Xiaoming Du and Paul Reeser and Suidan, {Makram T.} and Tiehong Huang and Moustafa Moteleb and Boufadel, {Michael C.} and Venosa, {Albert D.}",

year = "2005",

language = "English (US)",

pages = "5979--5984",

note = "2005 International Oil Spill Conference, IOSC 2005 ; Conference date: 15-05-2005 Through 19-05-2005",

}

TY - CONF

T1 - Optimum nitrogen concentration supporting maximum crude oil biodegradation in microcosms

AU - Du, Xiaoming

AU - Reeser, Paul

AU - Suidan, Makram T.

AU - Huang, Tiehong

AU - Moteleb, Moustafa

AU - Boufadel, Michael C.

AU - Venosa, Albert D.

PY - 2005

Y1 - 2005

N2 - The purpose of this study was to determine the minimum nitrogen concentration needed by microorganisms to biodegrade crude oil hydrocarbons attached to sand particles in a microcosm simulating a low energy marine beach. The experimental approach involved mixing clean sand with weathered Alaska North Slope crude oil and monitoring biodegradation in continuous-flow beach microcosms over a 40-day time period. Two experiments were conducted with different nitrate-N concentrations in the feed (0, 0.25, 0.5, 1.0, 2.5, 5.0 mg/L in the first experiment and 2.5, 5.0, 10, 25, and 50 mg/L in the second). Experimental results indicated that nitrate concentrations below approximately 10 mg/L limited the rate of crude oil biodegradation. This conclusion was supported by oxygen uptake, CO 2 production, nitrate consumption, and biomass growth data.

AB - The purpose of this study was to determine the minimum nitrogen concentration needed by microorganisms to biodegrade crude oil hydrocarbons attached to sand particles in a microcosm simulating a low energy marine beach. The experimental approach involved mixing clean sand with weathered Alaska North Slope crude oil and monitoring biodegradation in continuous-flow beach microcosms over a 40-day time period. Two experiments were conducted with different nitrate-N concentrations in the feed (0, 0.25, 0.5, 1.0, 2.5, 5.0 mg/L in the first experiment and 2.5, 5.0, 10, 25, and 50 mg/L in the second). Experimental results indicated that nitrate concentrations below approximately 10 mg/L limited the rate of crude oil biodegradation. This conclusion was supported by oxygen uptake, CO 2 production, nitrate consumption, and biomass growth data.

UR - http://www.scopus.com/inward/record.url?scp=33646030624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646030624&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33646030624

SP - 5979

EP - 5984

T2 - 2005 International Oil Spill Conference, IOSC 2005

Y2 - 15 May 2005 through 19 May 2005

ER -

Optimum nitrogen concentration supporting maximum crude oil biodegradation in microcosms

Abstract

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All Science Journal Classification (ASJC) codes

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