Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium

Na Liu, Haijun Li, Mengyan Li, Longzhen Ding, Chih Huang Weng, Cheng Di Dong

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.

Original languageEnglish (US)
Pages (from-to)98-105
Number of pages8
JournalBioresource Technology
Volume240
DOIs
StatePublished - Jan 1 2017

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Keywords

  • Dehalococcoides mccartyi
  • Non-dechlorinators
  • Oxygen
  • Trichloroethylene

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