Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium

Na Liu; Haijun Li; Mengyan Li; Longzhen Ding; Chih Huang Weng; Cheng Di Dong

doi:10.1016/j.biortech.2017.02.112

Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium

Na Liu, Haijun Li, Mengyan Li, Longzhen Ding, Chih Huang Weng, Cheng Di Dong

Chemistry and Environmental Science

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.

Original language	English (US)
Pages (from-to)	98-105
Number of pages	8
Journal	Bioresource Technology
Volume	240
DOIs	https://doi.org/10.1016/j.biortech.2017.02.112
State	Published - 2017

All Science Journal Classification (ASJC) codes

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

Keywords

Dehalococcoides mccartyi
Non-dechlorinators
Oxygen
Trichloroethylene

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10.1016/j.biortech.2017.02.112

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title = "Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium",

abstract = "The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.",

keywords = "Dehalococcoides mccartyi, Non-dechlorinators, Oxygen, Trichloroethylene",

author = "Na Liu and Haijun Li and Mengyan Li and Longzhen Ding and Weng, {Chih Huang} and Dong, {Cheng Di}",

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year = "2017",

doi = "10.1016/j.biortech.2017.02.112",

language = "English (US)",

volume = "240",

pages = "98--105",

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AU - Liu, Na

AU - Li, Haijun

AU - Li, Mengyan

AU - Ding, Longzhen

AU - Weng, Chih Huang

AU - Dong, Cheng Di

PY - 2017

Y1 - 2017

N2 - The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.

AB - The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the non-dechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged.

KW - Dehalococcoides mccartyi

KW - Non-dechlorinators

KW - Oxygen

KW - Trichloroethylene

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Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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