Abstract
This study evaluates the influence of pH and temperature on the partition coefficient of β-glucosidase and total protein in order to determine the best conditions for the purification of the enzyme in poly(ethylene glycol) (PEG) 4000 (8%, w/w) and potassium phosphate salt (13%, w/w) aqueous two-phase systems. The partition coefficient increased with pH and temperature for the ranges investigated (i.e., pH: 6.0-7.5; temperature: 25-55 °C). Yield and selectivity were key criteria for the selection of the optimum separation condition for enzyme purification. The highest selectivity was achieved at a pH of 6.5 and a temperature of 25 °C. A β-glucosidase yield greater than 92% was obtained in the bottom phase for pH values less than 7.0 and temperatures below 35 °C.
Original language | English (US) |
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Pages (from-to) | 104-108 |
Number of pages | 5 |
Journal | Biochemical Engineering Journal |
Volume | 30 |
Issue number | 1 |
DOIs | |
State | Published - May 1 2006 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Environmental Engineering
- Bioengineering
- Biomedical Engineering
Keywords
- Aqueous two-phase system
- Bioseparations
- Partition coefficient
- Protein
- Purification
- β-d-Glucosidase