Partitioning of β-glucosidase from Trichoderma reesei in poly(ethylene glycol) and potassium phosphate aqueous two-phase systems: Influence of pH and temperature

Shalini Gautam, Laurent Simon

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47 Scopus citations

Abstract

This study evaluates the influence of pH and temperature on the partition coefficient of β-glucosidase and total protein in order to determine the best conditions for the purification of the enzyme in poly(ethylene glycol) (PEG) 4000 (8%, w/w) and potassium phosphate salt (13%, w/w) aqueous two-phase systems. The partition coefficient increased with pH and temperature for the ranges investigated (i.e., pH: 6.0-7.5; temperature: 25-55 °C). Yield and selectivity were key criteria for the selection of the optimum separation condition for enzyme purification. The highest selectivity was achieved at a pH of 6.5 and a temperature of 25 °C. A β-glucosidase yield greater than 92% was obtained in the bottom phase for pH values less than 7.0 and temperatures below 35 °C.

Original languageEnglish (US)
Pages (from-to)104-108
Number of pages5
JournalBiochemical Engineering Journal
Volume30
Issue number1
DOIs
StatePublished - May 1 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Keywords

  • Aqueous two-phase system
  • Bioseparations
  • Partition coefficient
  • Protein
  • Purification
  • β-d-Glucosidase

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