Perfusion seeding of channeled elastomeric scaffolds with myocytes and endothelial cells for cardiac tissue engineering

Robert Maidhof, Anna Marsano, Eun Jung Lee, Gordana Vunjak-Novakovic

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

The requirements for engineering clinically sized cardiac constructs include medium perfusion (to maintain cell viability throughout the construct volume) and the protection of cardiac myocytes from hydrodynamic shear. To reconcile these conflicting requirements, we proposed the use of porous elastomeric scaffolds with an array of channels providing conduits for medium perfusion, and sized to provide efficient transport of oxygen to the cells, by a combination of convective flow and molecular diffusion over short distances between the channels. In this study, we investigate the conditions for perfusion seeding of channeled constructs with myocytes and endothelial cells without the gel carrier we previously used to lock the cells within the scaffold pores. We first established the flow parameters for perfusion seeding of porous elastomer scaffolds using the C2C12 myoblast line, and determined that a linear perfusion velocity of 1.0 mm/s resulted in seeding efficiency of 87% 26% within 2 hours. When applied to seeding of channeled scaffolds with neonatal rat cardiac myocytes, these conditions also resulted in high efficiency (77.2% 23.7%) of cell seeding. Uniform spatial cell distributions were obtained when scaffolds were stacked on top of one another in perfusion cartridges, effectively closing off the channels during perfusion seeding. Perfusion seeding of single scaffolds resulted in preferential cell attachment at the channel surfaces, and was employed for seeding scaffolds with rat aortic endothelial cells. We thus propose that these techniques can be utilized to engineer thick and compact cardiac constructs with parallel channels lined with endothelial cells.

Original languageEnglish (US)
Pages (from-to)565-572
Number of pages8
JournalBiotechnology Progress
Volume26
Issue number2
DOIs
StatePublished - Mar 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology

Keywords

  • Bioreactor
  • Cardiac tissue engineering
  • Cell seeding
  • Perfusion
  • Scaffold

Fingerprint

Dive into the research topics of 'Perfusion seeding of channeled elastomeric scaffolds with myocytes and endothelial cells for cardiac tissue engineering'. Together they form a unique fingerprint.

Cite this