Plasmonic gratings with nano-protrusions made by glancing angle deposition for single-molecule super-resolution imaging

B. Chen, A. Wood, A. Pathak, J. Mathai, S. Bok, H. Zheng, S. Hamm, S. Basuray, S. Grant, K. Gangopadhyay, P. V. Cornish, S. Gangopadhyay

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Super-resolution imaging has been advantageous in studying biological and chemical systems, but the required equipment and platforms are expensive and unable to observe single-molecules at the high (μM) fluorophore concentrations required to study protein interaction and enzymatic activity. Here, a plasmonic platform was designed that utilized an inexpensively fabricated plasmonic grating in combination with a scalable glancing angle deposition (GLAD) technique using physical vapor deposition. The GLAD creates an abundance of plasmonic nano-protrusion probes that combine the surface plasmon resonance (SPR) from the periodic gratings with the localized SPR of these nano-protrusions. The resulting platform enables simultaneous imaging of a large area without point-by-point scanning or bulk averaging for the detection of single Cyanine-5 molecules in dye concentrations ranging from 50 pM to 10 μM using epifluorescence microscopy. Combining the near-field plasmonic nano-protrusion probes and super-resolution technique using localization microscopy, we demonstrate the ability to resolve grain sizes down to 65 nm. This plasmonic GLAD grating is a cost-effective super-resolution imaging substrate with potential applications in high-speed biomedical imaging over a wide range of fluorescent concentrations.

Original languageEnglish (US)
Pages (from-to)12189-12201
Number of pages13
JournalNanoscale
Volume8
Issue number24
DOIs
StatePublished - Jun 28 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Fingerprint

Dive into the research topics of 'Plasmonic gratings with nano-protrusions made by glancing angle deposition for single-molecule super-resolution imaging'. Together they form a unique fingerprint.

Cite this