Predifferentiation of human MSCs in vitro leads to osteogenesis in vivo

S. J. Peter, T. Livingston, S. Gordon, G. Hebert, M. ElKalay, S. Kadiyala

Research output: Contribution to journalConference articlepeer-review


Mesenchymal stem cell (MSC)-loaded matrices were subjected to various in vitro differentiation regimes, followed by ectopic implantation in SCID mice. Sacrefices were performed at 2, 4, and 6 weeks and samples were processed for standard decalcified paraffin histology. Histologic analysis was performed in a blinded manner on samples explanted after six weeks. Gelfoam-MSC matrices that had been precultured for 8 to 14 days prior to implantation had more bony material than other constructs, although bone formation also occurred in these matrices under Dex culture conditions. Furthermore, the de novo bone formation was a result of the implanted cells.

Original languageEnglish (US)
Pages (from-to)236
Number of pages1
JournalASAIO Journal
Issue number2
StatePublished - 2000
Externally publishedYes
Event46th Annual Conference and Exposition of ASAIO - New York, NY, USA
Duration: Jun 28 2000Jul 1 2000

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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