Mesenchymal stem cell (MSC)-loaded matrices were subjected to various in vitro differentiation regimes, followed by ectopic implantation in SCID mice. Sacrefices were performed at 2, 4, and 6 weeks and samples were processed for standard decalcified paraffin histology. Histologic analysis was performed in a blinded manner on samples explanted after six weeks. Gelfoam-MSC matrices that had been precultured for 8 to 14 days prior to implantation had more bony material than other constructs, although bone formation also occurred in these matrices under Dex culture conditions. Furthermore, the de novo bone formation was a result of the implanted cells.
|Original language||English (US)|
|Number of pages||1|
|State||Published - 2000|
|Event||46th Annual Conference and Exposition of ASAIO - New York, NY, USA|
Duration: Jun 28 2000 → Jul 1 2000
All Science Journal Classification (ASJC) codes
- Biomedical Engineering