Protein loading, elution, and resolution behavior in a novel device that integrates ultrafiltration and chromatographic separation

Xiao Ping Dai, Sudipto Majumdar, Robert G. Luo, Kamalesh Sirkar

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Hollow fiber membranes and chromatographic resin beads are commonly employed in a variety of bioseparation processes. A new class of integrated separation devices is being studied in which the shell side of a hollow fiber device is filled with adsorbents/ chromatographic resin beads. Such devices and the corresponding separation methods integrate feed broth clarification by the microfiltration/ultrafiltration membrane with bioproduct purification by the shell-side resin beads either as an adsorbent or as beads in elution chromatography. A mathematical model has been developed for the prediction of the chromatographic behavior of such an integrated device. Simulations have been done to study the effects of axial dispersion, feed flow rate, water permeation rate, fiber packing density, and void fraction. Numerical solutions were obtained by solving the governing equations. This model can reasonably describe the concentration profiles as well as the breakthrough and elution behaviors in the integrated device.

Original languageEnglish (US)
Pages (from-to)125-139
Number of pages15
JournalBiotechnology and Bioengineering
Volume83
Issue number2
DOIs
StatePublished - Jul 20 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Keywords

  • Hollow fiber ultrafiltration membrane
  • Integrated membrane-chromatography model
  • Ion-exchange resin beads
  • Protein elution
  • Protein loading

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