Quantifying the membrane potential during E. coli growth stages

Corina Teodora Bot, Camelia Prodan

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The presence of the resting membrane potential has a strong effect on the dielectric behavior of cell suspensions. Using this observation and a well-established theoretical model, the low frequency dielectric dispersion curves of E. coli cell suspensions are de-convoluted to obtain the resting membrane potential of E. coli cells at various growth stages. Four regions of the exponential growth stage are investigated and the measurements indicate that the membrane depolarizes from - 220 mV in the early exponential phase to - 140 mV in the late exponential phase. The conductivity of the cell suspension is also found to decrease as the cells progress from the early to the late exponential phases.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalBiophysical Chemistry
Volume146
Issue number2-3
DOIs
StatePublished - Feb 1 2010

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Organic Chemistry

Keywords

  • Dielectric spectroscopy
  • Escherichia coli
  • Impedance analysis
  • Membrane potential

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