Reduction of 2,4-dichlorophenol toxicity to Pseudomonas putida after oxidative incubation with humic substances and a biomimetic catalyst

Dittmar Hahn, Annunziata Cozzolino, Alessandro Piccolo, Piero M. Armenante

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The effect of a synthetic iron(III)-porphyrin meso-tetra(2,6-dichloro-3-sulfonatophenyl)porphyrinate as a biomimetic catalyst in the oxidative treatment of 2,4-dichlorophenol (2,4-DCP) with humic substances and H2O2 was evaluated in factorial design experiments conducted at different concentrations of 2,4-DCP (0-25 ppm) and different incubation treatment times (0, 24, 96, or 120 h). In the absence of this treatment, bioassays with the bacterium Pseudomonas putida (ATCC11250) showed decreasing specific growth rates μ (used here to quantify 2,4-DCP toxicity) with increasing concentrations of 2,4-DCP. However, when 2,4-DCP was treated as mentioned above the toxicity of the resulting 2,4-DCP solution was reduced significantly. At low 2,4-DCP concentrations (up to 5 ppm) and long incubation periods (as long as 120 h), the specific growth rate μ was comparable to that of cultures grown in the absence of 2,4-DCP. The reduction in toxicity was directly correlated to a decrease in the concentration of 2,4-DCP in the treated solutions, as measured by high-performance liquid chromatography. The reduced concentrations of 2,4-DCP in the treated solutions could be correctly predicted based on the relationship between the specific growth rates and the 2,4-DCP concentrations in untreated solutions. These results indicate that the oxidative coupling of 2,4-DCP to humic substances catalyzed by the synthetic iron(III)-porphyrin catalyst in the presence of H2O2 is responsible for the removal of 2,4-DCP from solutions. This approach appears to be a promising alternative treatment to reduce 2,4-DCP bioavailability and thus toxicity in the environment.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalEcotoxicology and Environmental Safety
Volume66
Issue number3
DOIs
StatePublished - Mar 2007

All Science Journal Classification (ASJC) codes

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Keywords

  • 2
  • 4-DCP
  • Bioassay
  • Growth rate
  • Iron-porphyrin
  • Optical density
  • Respiration

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