Scalable nano-bioprobes with sub-cellular resolution for cell detection

Alokik Kanwal; Shanmugamurthy Lakshmanan; Ashwini Bendiganavale; Corina T. Bot; Anitha Patlolla; Rahul Raj; Camelia Prodan; Zafar Iqbal; Gordon A. Thomas; Reginald C. Farrow

doi:10.1016/j.bios.2013.01.066

Scalable nano-bioprobes with sub-cellular resolution for cell detection

Alokik Kanwal, Shanmugamurthy Lakshmanan, Ashwini Bendiganavale, Corina T. Bot, Anitha Patlolla, Rahul Raj, Camelia Prodan, Zafar Iqbal, Gordon A. Thomas, Reginald C. Farrow

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Here we present a carbon nanotube based device to noninvasively and quickly detect mobile single cells with the potential to maintain a high degree of spatial resolution. The device utilizes standard complementary metal oxide semiconductor (CMOS) technologies for fabrication, allowing it to be easily scalable (down to a few nanometers). Nanotubes are deposited using electrophoresis after fabrication in order to maintain CMOS compatibility. The devices are spaced by 6. μm which is the same size or smaller than a single cell. To demonstrate its capability to detect cells, we performed impedance spectroscopy on mobile human embryonic kidney (HEK) cells, neurons cells from mice, and yeast cells (S. pombe). Measurements were performed with and without cells and with and without nanotubes. Nanotubes were found to be crucial to successfully detect the presence of cells. The devices are also able to distinguish between cells with different characteristics.

Original language	English (US)
Pages (from-to)	267-273
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	45
Issue number	1
DOIs	https://doi.org/10.1016/j.bios.2013.01.066
State	Published - Jul 5 2013

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

Keywords

Bioprobes
Carbon nanotube
Cell detection
Cell identification

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10.1016/j.bios.2013.01.066

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title = "Scalable nano-bioprobes with sub-cellular resolution for cell detection",

abstract = "Here we present a carbon nanotube based device to noninvasively and quickly detect mobile single cells with the potential to maintain a high degree of spatial resolution. The device utilizes standard complementary metal oxide semiconductor (CMOS) technologies for fabrication, allowing it to be easily scalable (down to a few nanometers). Nanotubes are deposited using electrophoresis after fabrication in order to maintain CMOS compatibility. The devices are spaced by 6. μm which is the same size or smaller than a single cell. To demonstrate its capability to detect cells, we performed impedance spectroscopy on mobile human embryonic kidney (HEK) cells, neurons cells from mice, and yeast cells (S. pombe). Measurements were performed with and without cells and with and without nanotubes. Nanotubes were found to be crucial to successfully detect the presence of cells. The devices are also able to distinguish between cells with different characteristics.",

keywords = "Bioprobes, Carbon nanotube, Cell detection, Cell identification",

author = "Alokik Kanwal and Shanmugamurthy Lakshmanan and Ashwini Bendiganavale and Bot, {Corina T.} and Anitha Patlolla and Rahul Raj and Camelia Prodan and Zafar Iqbal and Thomas, {Gordon A.} and Farrow, {Reginald C.}",

note = "Funding Information: The authors would like to thank DARPA for funding the research ( FA9550-05-1-0461 , FA8650-08-1-7825 and N00014-08-1-0278 ) and Cornell Nanofabrication Facility (CNF) and its staff for the use of their cleanroom and expert advice during fabrication. The authors would also like to thank Prof. Andrew Hill and Kyle Dobiszewski for their help with the mice neurons, Dr. Jeffrey Fagan of the National Institute of Standards and Technology for providing the carbon nanotubes sorted by length, and Dr. Heiner Jaksch of Carl Zeiss for performing the high resolution SEM of the nanotubes deposited in the 200 nm spaced windows. ",

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AU - Kanwal, Alokik

AU - Lakshmanan, Shanmugamurthy

AU - Bendiganavale, Ashwini

AU - Bot, Corina T.

AU - Patlolla, Anitha

AU - Raj, Rahul

AU - Prodan, Camelia

AU - Iqbal, Zafar

AU - Thomas, Gordon A.

AU - Farrow, Reginald C.

N1 - Funding Information: The authors would like to thank DARPA for funding the research ( FA9550-05-1-0461 , FA8650-08-1-7825 and N00014-08-1-0278 ) and Cornell Nanofabrication Facility (CNF) and its staff for the use of their cleanroom and expert advice during fabrication. The authors would also like to thank Prof. Andrew Hill and Kyle Dobiszewski for their help with the mice neurons, Dr. Jeffrey Fagan of the National Institute of Standards and Technology for providing the carbon nanotubes sorted by length, and Dr. Heiner Jaksch of Carl Zeiss for performing the high resolution SEM of the nanotubes deposited in the 200 nm spaced windows.

PY - 2013/7/5

Y1 - 2013/7/5

N2 - Here we present a carbon nanotube based device to noninvasively and quickly detect mobile single cells with the potential to maintain a high degree of spatial resolution. The device utilizes standard complementary metal oxide semiconductor (CMOS) technologies for fabrication, allowing it to be easily scalable (down to a few nanometers). Nanotubes are deposited using electrophoresis after fabrication in order to maintain CMOS compatibility. The devices are spaced by 6. μm which is the same size or smaller than a single cell. To demonstrate its capability to detect cells, we performed impedance spectroscopy on mobile human embryonic kidney (HEK) cells, neurons cells from mice, and yeast cells (S. pombe). Measurements were performed with and without cells and with and without nanotubes. Nanotubes were found to be crucial to successfully detect the presence of cells. The devices are also able to distinguish between cells with different characteristics.

AB - Here we present a carbon nanotube based device to noninvasively and quickly detect mobile single cells with the potential to maintain a high degree of spatial resolution. The device utilizes standard complementary metal oxide semiconductor (CMOS) technologies for fabrication, allowing it to be easily scalable (down to a few nanometers). Nanotubes are deposited using electrophoresis after fabrication in order to maintain CMOS compatibility. The devices are spaced by 6. μm which is the same size or smaller than a single cell. To demonstrate its capability to detect cells, we performed impedance spectroscopy on mobile human embryonic kidney (HEK) cells, neurons cells from mice, and yeast cells (S. pombe). Measurements were performed with and without cells and with and without nanotubes. Nanotubes were found to be crucial to successfully detect the presence of cells. The devices are also able to distinguish between cells with different characteristics.

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Scalable nano-bioprobes with sub-cellular resolution for cell detection

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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