Self-Assembly of a Dentinogenic Peptide Hydrogel

Peter K. Nguyen, William Gao, Saloni D. Patel, Zain Siddiqui, Saul Weiner, Emi Shimizu, Biplab Sarkar, Vivek A. Kumar

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


Current standard of care for treating infected dental pulp, root canal therapy, retains the physical properties of the tooth to a large extent, but does not aim to rejuvenate the pulp tissue. Tissue-engineered acellular biomimetic hydrogels have great potential to facilitate the regeneration of the tissue through the recruitment of autologous stem cells. We propose the use of a dentinogenic peptide that self-assembles into β-sheet-based nanofibers that constitute a biodegradable and injectable hydrogel for support of dental pulp stem cells. The peptide backbone contains a β-sheet-forming segment and a matrix extracellular phosphoglycoprotein mimic sequence at the C-terminus. The high epitope presentation of the functional moiety in the self-assembled nanofibers may enable recapitulation of a functional niche for the survival and proliferation of autologous cells. We elucidated the hierarchical self-assembly of the peptide through biophysical techniques, including scanning electron microscopy and atomic force microscopy. The material property of the self-assembled hydrogel was probed though oscillatory rheometry, demonstrating its thixotropic nature. We also demonstrate the cytocompatibility of the hydrogel with respect to fibroblasts and dental pulp stem cells. The self-assembled peptide platform holds promise for guided dentinogenesis and it can be tailored to a variety of applications in soft tissue engineering and translational medicine in the future.

Original languageEnglish (US)
Pages (from-to)5980-5987
Number of pages8
JournalACS Omega
Issue number6
StatePublished - Jun 30 2018

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Chemical Engineering


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