@article{305e9ee7bc604747bbc4feddbb8870c9,
title = "Semaphorin signaling restricts neuronal regeneration in C. elegans",
abstract = "Extracellular signaling proteins serve as neuronal growth cone guidance molecules during development and are well positioned to be involved in neuronal regeneration and recovery from injury. Semaphorins and their receptors, the plexins, are a family of conserved proteins involved in development that, in the nervous system, are axonal guidance cues mediating axon pathfinding and synapse formation. The Caenorhabditis elegans genome encodes for three semaphorins and two plexin receptors: the transmembrane semaphorins, SMP-1 and SMP-2, signal through their receptor, PLX-1, while the secreted semaphorin, MAB-20, signals through PLX-2. Here, we evaluate the locomotion behavior of knockout animals missing each of the semaphorins and plexins and the neuronal morphology of plexin knockout animals; we described the cellular expression pattern of the promoters of all plexins in the nervous system of C. elegans; and we evaluated their effect on the regrowth and reconnection of motoneuron neurites and the recovery of locomotion behavior following precise laser microsurgery. Regrowth and reconnection were more prevalent in the absence of each plexin, while recovery of locomotion surpassed regeneration in all genotypes.",
keywords = "C. elegans, laser microsurgery, locomotion, neuroregeneration, plexins, regeneration, semaphorins",
author = "Harreguy, {Maria B.} and Zainab Tanvir and Esha Shah and Blandine Simprevil and Tran, {Tracy S.} and Gal Haspel",
note = "Funding Information: We thank Joseph Culotti (University of Toronto, Mt Sinai Hospital) and Ricard Ikegami (UC Berkeley) for sharing the plx-2 and plx-1 reporter strains. We also thank Kang Shen (Stanford University) and Kota Mizumoto (University of British Columbia) for the plx-1 reporter constructs. We thank Monica Driscoll (Rutgers University) for her help in generating transgenic lines and the Hobert Lab (Columbia University) for the NeuroPal strains. We thank Joseph Soubany for help with locomotion anaysis. Research reported in this publication was supported by NINDS of the National Institutes of Health (1R15NS125565-01; MBH, SE, SB, TST, and GH), by the State of New Jersey Commission on Spinal Cord Research (CSCR14ERG002; GH; and CSCR16IRG013; TST), by IOS of the National Science Foundation (2034864; TST), and by the NJIT Undergraduate Research Initiative (URI; ES). Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). Funding Information: We thank Joseph Culotti ( University of Toronto, Mt Sinai Hospital) and Ricard Ikegami (UC Berkeley) for sharing the plx-2 and plx-1 reporter strains. We also thank Kang Shen (Stanford University) and Kota Mizumoto (University of British Columbia) for the plx-1 reporter constructs. We thank Monica Driscoll (Rutgers University) for her help in generating transgenic lines and the Hobert Lab (Columbia University) for the NeuroPal strains. We thank Joseph Soubany for help with locomotion anaysis. Research reported in this publication was supported by NINDS of the National Institutes of Health (1R15NS125565-01; MBH, SE, SB, TST, and GH), by the State of New Jersey Commission on Spinal Cord Research (CSCR14ERG002; GH; and CSCR16IRG013; TST), by IOS of the National Science Foundation (2034864; TST), and by the NJIT Undergraduate Research Initiative (URI; ES). Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). Publisher Copyright: Copyright {\textcopyright} 2022 Harreguy, Tanvir, Shah, Simprevil, Tran and Haspel.",
year = "2022",
month = oct,
day = "17",
doi = "10.3389/fcell.2022.814160",
language = "English (US)",
volume = "10",
journal = "Frontiers in Cell and Developmental Biology",
issn = "2296-634X",
publisher = "Frontiers Media S. A.",
}