Study of electrochemical reactions using nanospray desorption electrospray ionization mass spectrometry

Pengyuan Liu, Ingela T. Lanekoff, Julia Laskin, Howard D. Dewald, Hao Chen

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The combination of electrochemistry (EC) and mass spectrometry (MS) is a powerful analytical tool for studying mechanisms of redox reactions, identification of products and intermediates, and online derivatization/ recognition of analytes. This work reports a new coupling interface for EC/MS by employing nanospray desorption electrospray ionization, a recently developed ambient ionization method. We demonstrate online coupling of nanospray desorption electrospray ionization MS with a traditional electrochemical flow cell, in which the electrolyzed solution emanating from the cell is ionized by nanospray desorption electrospray ionization for MS analysis. Furthermore, we show first coupling of nanospray desorption electrospray ionization MS with an interdigitated array (IDA) electrode enabling chemical analysis of electrolyzed samples directly from electrode surfaces. Because of its inherent sensitivity, nanospray desorption electrospray ionization enables chemical analysis of small volumes and concentrations of sample solution. Specifically, good-quality signal of dopamine and its oxidized form, dopamine o-quinone, was obtained using 10 μL of 1 μM solution of dopamine on the IDA. Oxidation of dopamine, reduction of benzodiazepines, and electrochemical derivatization of thiol groups were used to demonstrate the performance of the technique. Our results show the potential of nanospray desorption electrospray ionization as a novel interface for electrochemical mass spectrometry research.

Original languageEnglish (US)
Pages (from-to)5737-5743
Number of pages7
JournalAnalytical Chemistry
Volume84
Issue number13
DOIs
StatePublished - Jul 3 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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