Substrate effect on dynamic indentation measurement of biological cell properties

Guoxin Cao, Namas Chandra

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Viscoelastic mechanical properties of biological cells are commonly measured using atomic force microscope (AFM) dynamic indentation method with spherical tips. Storage and loss modulii of cells are then computed from the indentation force-displacement response under dynamic loading conditions. It is shown in current numerical simulations that those modulii computed based on existing analysis can not reflect the true values due to the substrate effect. This effect can alter the indentation modulus by changing the geometric relations between the indentation displacement and the contact area. Typically, the cell modulii are significantly overestimated in the existing indentation analysis.

Original languageEnglish (US)
Title of host publicationStructure-Property Relationships in Biomineralized and Biomimetic Composites
PublisherMaterials Research Society
Pages121-126
Number of pages6
ISBN (Print)9781605111605
DOIs
StatePublished - 2009
Externally publishedYes
Event2009 MRS Spring Meeting - San Francisco, CA, United States
Duration: Apr 14 2009Apr 17 2009

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1187
ISSN (Print)0272-9172

Other

Other2009 MRS Spring Meeting
Country/TerritoryUnited States
CitySan Francisco, CA
Period4/14/094/17/09

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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