Surface micromachined dielectrophoretic gates for the front-end device of a biodetection system

Conrad D. James, Murat Okandan, Paul Galambos, Seethambal S. Mani, Dawn Bennett, Boris Khusid, Andreas Acrivos

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We present a novel separation device for the front-end of a biodetection system to discriminate between biological and non-biological analytes captured in air samples. By combining AC dielectrophoresis along the flow streamlines and a field-induced phase-separation, the device utilizes "dielectrophoretic gating" to separate analytes suspended in a flowing fluid based on their intrinsic polarizability properties. The gates are integrated into batch fabricated self-sealed surface-micromachined fluid channels. We demonstrate that setting the gate to a moderate voltage in the radio frequency range removed bacteria cells from a mixture containing non-biological particles without the need for fluorescent labeling or antibody-antigen hybridization, and also validate experimentally basic relations for estimating the gate performance.

Original languageEnglish (US)
Pages (from-to)14-19
Number of pages6
JournalJournal of Fluids Engineering, Transactions of the ASME
Volume128
Issue number1
DOIs
StatePublished - Jan 1 2006

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering

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