TY - JOUR
T1 - Synaptic dynamics convey differential sensitivity to input pattern changes in two muscles innervated by the same motor neurons
AU - Daur, Nelly
AU - Nadim, Farzan
AU - Bucher, Dirk
N1 - Publisher Copyright:
© 2021 Daur et al.
PY - 2021/11/1
Y1 - 2021/11/1
N2 - Postsynaptic responses depend on input patterns as well as short-term synaptic plasticity, summation, and postsynaptic membrane properties, but the interactions of those dynamics with realistic input patterns are not well understood. We recorded the responses of the two pyloric dilator (PD) muscles, cpv2a and cpv2b, that are innervated by and receive identical periodic bursting input from the same two motor neurons in the lobster Homarus americanus. Cpv2a and cpv2b showed quantitative differences in membrane nonlinearities and synaptic summation. At a short timescale, responses in both muscles were dominated by facilitation, albeit with different frequency and time dependence. Realistic burst stimulations revealed more substantial differences. Across bursts, cpv2a showed transient depression, whereas cpv2b showed transient facilitation. Steady-state responses to bursting input also differed substantially. Neither muscle had a monotonic dependence on frequency, but cpv2b showed particularly pronounced bandpass filtering. Cpv2a was sensitive to changes in both burst frequency and intra-burst spike frequency, whereas, despite its much slower responses, cpv2b was largely insensitive to changes in burst frequency. Cpv2a was sensitive to both burst duration and number of spikes per burst, whereas cpv2b was sensitive only to the former parameter. Neither muscle showed consistent sensitivity to changes in the overall spike interval structure, but cpv2b was surprisingly sensitive to changes in the first intervals in each burst, a parameter known to be regulated by dopamine (DA) modulation of spike propagation of the presynaptic axon. These findings highlight how seemingly minor circuit output changes mediated by neuromodulation could be read out differentially at the two synapses.
AB - Postsynaptic responses depend on input patterns as well as short-term synaptic plasticity, summation, and postsynaptic membrane properties, but the interactions of those dynamics with realistic input patterns are not well understood. We recorded the responses of the two pyloric dilator (PD) muscles, cpv2a and cpv2b, that are innervated by and receive identical periodic bursting input from the same two motor neurons in the lobster Homarus americanus. Cpv2a and cpv2b showed quantitative differences in membrane nonlinearities and synaptic summation. At a short timescale, responses in both muscles were dominated by facilitation, albeit with different frequency and time dependence. Realistic burst stimulations revealed more substantial differences. Across bursts, cpv2a showed transient depression, whereas cpv2b showed transient facilitation. Steady-state responses to bursting input also differed substantially. Neither muscle had a monotonic dependence on frequency, but cpv2b showed particularly pronounced bandpass filtering. Cpv2a was sensitive to changes in both burst frequency and intra-burst spike frequency, whereas, despite its much slower responses, cpv2b was largely insensitive to changes in burst frequency. Cpv2a was sensitive to both burst duration and number of spikes per burst, whereas cpv2b was sensitive only to the former parameter. Neither muscle showed consistent sensitivity to changes in the overall spike interval structure, but cpv2b was surprisingly sensitive to changes in the first intervals in each burst, a parameter known to be regulated by dopamine (DA) modulation of spike propagation of the presynaptic axon. These findings highlight how seemingly minor circuit output changes mediated by neuromodulation could be read out differentially at the two synapses.
KW - Bursting neuron
KW - Depression
KW - Facilitation
KW - Frequency filtering
KW - Short-term synaptic plasticity
KW - Stomatogastric
UR - http://www.scopus.com/inward/record.url?scp=85119612596&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85119612596&partnerID=8YFLogxK
U2 - 10.1523/ENEURO.0351-21.2021
DO - 10.1523/ENEURO.0351-21.2021
M3 - Article
C2 - 34764189
AN - SCOPUS:85119612596
SN - 2373-2822
VL - 8
JO - eNeuro
JF - eNeuro
IS - 6
M1 - ENEURO.0351-21.2021
ER -