@inbook{a1c52ef218624dd79a8ae5a969f9eee9,
title = "Synthesis of PLGA–lipid hybrid nanoparticles for siRNA delivery using the emulsion method PLGA-PEG–lipid nanoparticles for siRNA delivery",
abstract = "The effective delivery of small interfering RNA (siRNA) to tumor cells remains a challenge for applications in cancer therapy. The development of polymeric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG–cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo.",
keywords = "Cationic lipid, Double emulsion solvent evaporation, PLGA-PEG polymer, Polymeric nanoparticles, siRNA delivery",
author = "Lei Wang and Benjamin Griffel and Xiaoyang Xu",
note = "Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",
year = "2017",
doi = "10.1007/978-1-4939-7138-1_15",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "231--240",
booktitle = "Methods in Molecular Biology",
}