Synthesis of PLGA–lipid hybrid nanoparticles for siRNA delivery using the emulsion method PLGA-PEG–lipid nanoparticles for siRNA delivery

Lei Wang, Benjamin Griffel, Xiaoyang Xu

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

The effective delivery of small interfering RNA (siRNA) to tumor cells remains a challenge for applications in cancer therapy. The development of polymeric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG–cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages231-240
Number of pages10
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1632
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Cationic lipid
  • Double emulsion solvent evaporation
  • PLGA-PEG polymer
  • Polymeric nanoparticles
  • siRNA delivery

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