Abstract
Evidence from amino acid composition, Fourier transform analysis of primary structure and secondary structure prediction suggests a tripartite structure for Ceratitis capitata eggshell proteins Ccs36 and Ccs38, which consists of a central domain and two flanking 'arms'. The proteins, apparently, contain tandemly repeating peptide motifs specific for each domain of the tripartite structure. The central domain of both proteins, which exhibits extensive sequence homology with the corresponding domains of Drosophila melanogaster proteins s36 and s38, is formed by tandem repeats of an octapeptide-X-X-X-Z-Z-Z-Z-Z- (where X= large hydrophobic residue and Z = β-turn former residue) and its variants. It is predicted to adopt a compact, most probably twisted, antiparallel β-pleated sheet structure of β-sheet strands regularly alternating with β-turns or loops. The central domains of Ccs36 and Ccs38 share structural similarities, but they are recognizably different. The 'arms' of the proteins presumably serving for protein and species-specific functions differ substantially from those of Drosophila melanogaster. In Ccs36, the C-terminal 'arm' is formed by, almost precise, tandem repeats of an octapeptide -Y-X-A-A-P-A-A-S- (X = G or S), whereas the N-terminal 'arm' contains repeats of the predicted, alternating with β-turns. In the Ccs38 C-terminal 'arm' nonapeptide repeats of the form -Y-Z-Z-Z-Z-(G,A)-Z-Q-Z-(Z = A, G, P or S) are observed, whereas the N-terminal includes repeats of an octapeptide motif -x-y-G-z-G-u-G-v- (x = I,S, y = G, Q). The latter are predicted as β-sheet strands alternating with β-turns, whereas, for the former the evidence is conflicting. The presence of these motifs suggests periodical patterns of dityrosine crosslinks, which harden the eggshell rendering it insoluble. Fourier transform infrared spectroscopy data from intact Ceratitis capitata eggshells support the validity of prediction.
Original language | English (US) |
---|---|
Pages (from-to) | 307-315 |
Number of pages | 9 |
Journal | International Journal of Biological Macromolecules |
Volume | 13 |
Issue number | 5 |
DOIs | |
State | Published - Oct 1991 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Structural Biology
- Biochemistry
- Molecular Biology
- Economics and Econometrics
- General Energy
Keywords
- Ceratitis capitata
- Fourier analysis
- eggshell (chorion) protein structure
- infrared spectroscopy
- secondary structure prediction
- tandemly repeating peptide motifs