Target discovery of ebselen with a biotinylated probe

Zhenzhen Chen; Zhongyao Jiang; Nan Chen; Qian Shi; Lili Tong; Fanpeng Kong; Xiufen Cheng; Hao Chen; Chu Wang; Bo Tang

doi:10.1039/c8cc04258f

Target discovery of ebselen with a biotinylated probe

Zhenzhen Chen, Zhongyao Jiang, Nan Chen, Qian Shi, Lili Tong, Fanpeng Kong, Xiufen Cheng, Hao Chen, Chu Wang, Bo Tang

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Despite numerous studies on ebselen over the past decade, its cellular targets remain obscure. Here we synthesized a biotinylated ebselen probe (biotin-ebselen) and characterized ebselen-binding proteins via an efficient activity-based protein profiling (ABPP) method, which allowed for the robust identification of 462 targeted proteins in HeLa cells. This first work of global target profiling of ebselen will be helpful to re-design ebselen-based therapy appropriately in clinical trials.

Original language	English (US)
Pages (from-to)	9506-9509
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	68
DOIs	https://doi.org/10.1039/c8cc04258f
State	Published - 2018
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/c8cc04258f

Cite this

@article{a073fb6e8199494eac5aad803704bd56,

title = "Target discovery of ebselen with a biotinylated probe",

abstract = "Despite numerous studies on ebselen over the past decade, its cellular targets remain obscure. Here we synthesized a biotinylated ebselen probe (biotin-ebselen) and characterized ebselen-binding proteins via an efficient activity-based protein profiling (ABPP) method, which allowed for the robust identification of 462 targeted proteins in HeLa cells. This first work of global target profiling of ebselen will be helpful to re-design ebselen-based therapy appropriately in clinical trials.",

author = "Zhenzhen Chen and Zhongyao Jiang and Nan Chen and Qian Shi and Lili Tong and Fanpeng Kong and Xiufen Cheng and Hao Chen and Chu Wang and Bo Tang",

note = "Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

year = "2018",

doi = "10.1039/c8cc04258f",

language = "English (US)",

volume = "54",

pages = "9506--9509",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "68",

}

TY - JOUR

T1 - Target discovery of ebselen with a biotinylated probe

AU - Chen, Zhenzhen

AU - Jiang, Zhongyao

AU - Chen, Nan

AU - Shi, Qian

AU - Tong, Lili

AU - Kong, Fanpeng

AU - Cheng, Xiufen

AU - Chen, Hao

AU - Wang, Chu

AU - Tang, Bo

PY - 2018

Y1 - 2018

N2 - Despite numerous studies on ebselen over the past decade, its cellular targets remain obscure. Here we synthesized a biotinylated ebselen probe (biotin-ebselen) and characterized ebselen-binding proteins via an efficient activity-based protein profiling (ABPP) method, which allowed for the robust identification of 462 targeted proteins in HeLa cells. This first work of global target profiling of ebselen will be helpful to re-design ebselen-based therapy appropriately in clinical trials.

AB - Despite numerous studies on ebselen over the past decade, its cellular targets remain obscure. Here we synthesized a biotinylated ebselen probe (biotin-ebselen) and characterized ebselen-binding proteins via an efficient activity-based protein profiling (ABPP) method, which allowed for the robust identification of 462 targeted proteins in HeLa cells. This first work of global target profiling of ebselen will be helpful to re-design ebselen-based therapy appropriately in clinical trials.

UR - http://www.scopus.com/inward/record.url?scp=85051953042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051953042&partnerID=8YFLogxK

U2 - 10.1039/c8cc04258f

DO - 10.1039/c8cc04258f

M3 - Article

C2 - 30091742

AN - SCOPUS:85051953042

SN - 1359-7345

VL - 54

SP - 9506

EP - 9509

JO - Chemical Communications

JF - Chemical Communications

IS - 68

ER -

Target discovery of ebselen with a biotinylated probe

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this