The chorion genes of the medfly, Ceratitis capitata. II. Characterization of three novel cDNA clones obtained by differential screening of an ovarian library

Peter P. Tolias; Mary Konsolaki; Katia Komitopoulou; Fotis C. Kafatos

doi:10.1016/0012-1606(90)90057-P

The chorion genes of the medfly, Ceratitis capitata. II. Characterization of three novel cDNA clones obtained by differential screening of an ovarian library

Peter P. Tolias, Mary Konsolaki, Katia Komitopoulou, Fotis C. Kafatos

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Abstract

We have isolated three new chorion cDNA clones from a Ceratitis capitata ovarian library. Their isolation was accomplished by differential screening of the library using as probes ³²P-labeled poly(A)⁺ mRNAs obtained from hand-staged medfly choriogenic versus prechoriogenic follicles. RNA blot hybridization analysis revealed that the genes corresponding to these clones have unique temporal profiles of mRNA accumulation, restricted to specific choriogenic stages. In addition, in vitro translation products encoded by these cDNAs approximately comigrated with polypeptides synthesized de novo in culture by choriogenic follicles. All three genes are located in regions of the medfly genome that are specifically amplified in female ovaries. DNA sequence analysis has revealed that one of these clones is derived from a homolog of the Drosophila melanogaster s38 chorion gene. It appears that, although D. melanogaster and C. capitata are separated by at least 120 million years of evolution, the mechanisms by which chorion genes are expressed and regulated during development have been well maintained. We suggest that the regulatory elements controlling the expression of sex-specific (e.g., chorion) genes may be isolated and used to construct transgenic medfly strains from which females could be eliminated by negative selection; such strains could be used as part of an effort to control this agricultural pest.

Original language	English (US)
Pages (from-to)	105-112
Number of pages	8
Journal	Developmental Biology
Volume	140
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(90)90057-P
State	Published - Jul 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/0012-1606(90)90057-P

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@article{4f500a2a24a8459ba70d51132749048b,

title = "The chorion genes of the medfly, Ceratitis capitata. II. Characterization of three novel cDNA clones obtained by differential screening of an ovarian library",

abstract = "We have isolated three new chorion cDNA clones from a Ceratitis capitata ovarian library. Their isolation was accomplished by differential screening of the library using as probes 32P-labeled poly(A)+ mRNAs obtained from hand-staged medfly choriogenic versus prechoriogenic follicles. RNA blot hybridization analysis revealed that the genes corresponding to these clones have unique temporal profiles of mRNA accumulation, restricted to specific choriogenic stages. In addition, in vitro translation products encoded by these cDNAs approximately comigrated with polypeptides synthesized de novo in culture by choriogenic follicles. All three genes are located in regions of the medfly genome that are specifically amplified in female ovaries. DNA sequence analysis has revealed that one of these clones is derived from a homolog of the Drosophila melanogaster s38 chorion gene. It appears that, although D. melanogaster and C. capitata are separated by at least 120 million years of evolution, the mechanisms by which chorion genes are expressed and regulated during development have been well maintained. We suggest that the regulatory elements controlling the expression of sex-specific (e.g., chorion) genes may be isolated and used to construct transgenic medfly strains from which females could be eliminated by negative selection; such strains could be used as part of an effort to control this agricultural pest.",

author = "Tolias, {Peter P.} and Mary Konsolaki and Katia Komitopoulou and Kafatos, {Fotis C.}",

note = "Funding Information: We thank N. Brown for guidance in the construction of the medfly ovarian cDNA library; M. Frisardi for providing the medfly PS2zcr gene used as a single copy control in gene amplification studies; A. Zacharopoulou for communicating unpublished results; and C. Swimmer, M. Frisardi, and other members of the Kafatos Laboratory at Harvard and of the IMBB in Crete for helpful comments. We also thank M. Youk-See for artwork; B. Klumpar for photography; and E. Fenerjian for secretarial assistance. P.P.T. was supported by a postdoctoral fellowship from the Medical Research Council of Canada. This work was supported by USDA and ACS grants (F.C.K.) and a grant from the Greek General Secretariat for Research and Technology (K.K. and F.C.K.). Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1990",

month = jul,

doi = "10.1016/0012-1606(90)90057-P",

language = "English (US)",

volume = "140",

pages = "105--112",

journal = "Developmental Biology",

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T1 - The chorion genes of the medfly, Ceratitis capitata. II. Characterization of three novel cDNA clones obtained by differential screening of an ovarian library

AU - Tolias, Peter P.

AU - Konsolaki, Mary

AU - Komitopoulou, Katia

AU - Kafatos, Fotis C.

N1 - Funding Information: We thank N. Brown for guidance in the construction of the medfly ovarian cDNA library; M. Frisardi for providing the medfly PS2zcr gene used as a single copy control in gene amplification studies; A. Zacharopoulou for communicating unpublished results; and C. Swimmer, M. Frisardi, and other members of the Kafatos Laboratory at Harvard and of the IMBB in Crete for helpful comments. We also thank M. Youk-See for artwork; B. Klumpar for photography; and E. Fenerjian for secretarial assistance. P.P.T. was supported by a postdoctoral fellowship from the Medical Research Council of Canada. This work was supported by USDA and ACS grants (F.C.K.) and a grant from the Greek General Secretariat for Research and Technology (K.K. and F.C.K.). Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1990/7

Y1 - 1990/7

N2 - We have isolated three new chorion cDNA clones from a Ceratitis capitata ovarian library. Their isolation was accomplished by differential screening of the library using as probes 32P-labeled poly(A)+ mRNAs obtained from hand-staged medfly choriogenic versus prechoriogenic follicles. RNA blot hybridization analysis revealed that the genes corresponding to these clones have unique temporal profiles of mRNA accumulation, restricted to specific choriogenic stages. In addition, in vitro translation products encoded by these cDNAs approximately comigrated with polypeptides synthesized de novo in culture by choriogenic follicles. All three genes are located in regions of the medfly genome that are specifically amplified in female ovaries. DNA sequence analysis has revealed that one of these clones is derived from a homolog of the Drosophila melanogaster s38 chorion gene. It appears that, although D. melanogaster and C. capitata are separated by at least 120 million years of evolution, the mechanisms by which chorion genes are expressed and regulated during development have been well maintained. We suggest that the regulatory elements controlling the expression of sex-specific (e.g., chorion) genes may be isolated and used to construct transgenic medfly strains from which females could be eliminated by negative selection; such strains could be used as part of an effort to control this agricultural pest.

AB - We have isolated three new chorion cDNA clones from a Ceratitis capitata ovarian library. Their isolation was accomplished by differential screening of the library using as probes 32P-labeled poly(A)+ mRNAs obtained from hand-staged medfly choriogenic versus prechoriogenic follicles. RNA blot hybridization analysis revealed that the genes corresponding to these clones have unique temporal profiles of mRNA accumulation, restricted to specific choriogenic stages. In addition, in vitro translation products encoded by these cDNAs approximately comigrated with polypeptides synthesized de novo in culture by choriogenic follicles. All three genes are located in regions of the medfly genome that are specifically amplified in female ovaries. DNA sequence analysis has revealed that one of these clones is derived from a homolog of the Drosophila melanogaster s38 chorion gene. It appears that, although D. melanogaster and C. capitata are separated by at least 120 million years of evolution, the mechanisms by which chorion genes are expressed and regulated during development have been well maintained. We suggest that the regulatory elements controlling the expression of sex-specific (e.g., chorion) genes may be isolated and used to construct transgenic medfly strains from which females could be eliminated by negative selection; such strains could be used as part of an effort to control this agricultural pest.

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JO - Developmental Biology

JF - Developmental Biology

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The chorion genes of the medfly, Ceratitis capitata. II. Characterization of three novel cDNA clones obtained by differential screening of an ovarian library

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