The E3 ubiquitin ligase adaptor Tango10 links the core circadian clock to neuropeptide and behavioral rhythms

Jongbin Lee, Chunghun Lim, Tae Hee Han, Tomas Andreani, Matthew Moye, Jack Curran, Eric Johnson, William L. Kath, Casey O. Diekman, Bridget C. Lear, Ravi Allada

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Circadian transcriptional timekeepers in pacemaker neurons drive profound daily rhythms in sleep and wake. Here we reveal a molecular pathway that links core transcriptional oscillators to neuronal and behavioral rhythms. Using two independent genetic screens, we identified mutants of Transport and Golgi organization 10 (Tango10) with poor behavioral rhythmicity. Tango10 expression in pacemaker neurons expressing the neuropeptide PIGMENT-DISPERSING FACTOR (PDF) is required for robust rhythms. Loss of Tango10 results in elevated PDF accumulation in nerve terminals even in mutants lacking a functional core clock. TANGO10 protein itself is rhythmically expressed in PDF terminals. Mass spectrometry of TANGO10 complexes reveals interactions with the E3 ubiquitin ligase CULLIN 3 (CUL3). CUL3 depletion phenocopies Tango10 mutant effects on PDF even in the absence of the core clock gene timeless. Patch clamp electrophysiology in Tango10 mutant neurons demonstrates elevated spontaneous firing potentially due to reduced voltage-gated Shaker-like potassium currents. We propose that Tango10/Cul3 transduces molecular oscillations from the core clock to neuropeptide release important for behavioral rhythms.

Original languageEnglish (US)
Article numbere2110767118
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number47
StatePublished - Nov 23 2021

All Science Journal Classification (ASJC) codes

  • General


  • Circadian rhythms
  • Drosophila
  • Neuronal output
  • Potassium current
  • Ubiquitin ligase


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