TY - JOUR
T1 - The KaiA protein of the cyanobacterial circadian oscillator is modulated by a redox-active cofactor
AU - Wood, Thammajun L.
AU - Bridwell-Rabb, Jennifer
AU - Kim, Yong Ick
AU - Gao, Tiyu
AU - Chang, Yong Gang
AU - LiWang, Andy
AU - Barondeau, David P.
AU - Golden, Susan S.
PY - 2010/3/30
Y1 - 2010/3/30
N2 - The circadian rhythms exhibited in the cyanobacterium Synechococcus elongatus are generated by an oscillator comprised of the proteins KaiA, KaiB, and KaiC. An external signal that commonly affects the circadian clock is light. Previously, we reported that the bacteriophytochrome-like protein CikA passes environmental signals to the oscillator by directly binding a quinone and using cellular redox state as a measure of light in this photosynthetic organism. Here, we report that KaiA also binds the quinone analog 2,5-dibromo-3-methyl-6- isopropyl-p-benzoquinone (DBMIB), and the oxidized form of DBMIB, but not its reduced form, decreases the stability of KaiA in vivo, causes multimerization in vitro, and blocks KaiA stimulation of KaiC phosphorylation, which is central to circadian oscillation. Our data suggest that KaiA directly senses environmental signals as changes in redox state and modulates the circadian clock.
AB - The circadian rhythms exhibited in the cyanobacterium Synechococcus elongatus are generated by an oscillator comprised of the proteins KaiA, KaiB, and KaiC. An external signal that commonly affects the circadian clock is light. Previously, we reported that the bacteriophytochrome-like protein CikA passes environmental signals to the oscillator by directly binding a quinone and using cellular redox state as a measure of light in this photosynthetic organism. Here, we report that KaiA also binds the quinone analog 2,5-dibromo-3-methyl-6- isopropyl-p-benzoquinone (DBMIB), and the oxidized form of DBMIB, but not its reduced form, decreases the stability of KaiA in vivo, causes multimerization in vitro, and blocks KaiA stimulation of KaiC phosphorylation, which is central to circadian oscillation. Our data suggest that KaiA directly senses environmental signals as changes in redox state and modulates the circadian clock.
KW - Biological rhythms
KW - DBMIB
KW - Environmental signals
KW - Pseudoreceiver
KW - Quinone
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U2 - 10.1073/pnas.0910141107
DO - 10.1073/pnas.0910141107
M3 - Article
C2 - 20231482
AN - SCOPUS:77950528207
SN - 0027-8424
VL - 107
SP - 5804
EP - 5809
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 13
ER -