The killer outfit and timing: Impact of the fabric and time in body fluid identification and DNA profiling

Sara C. Zapico, Valerie Lascano, Tarik Sadik, Proggya Paromita, Jenely Amaya, Christian Stadler, Gabriela Roca

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The present work aimed to study the detection, through lateral flow immunochromatographic (LFI) tests, of saliva samples over time in three different types of fabrics, as well as, the possibility of DNA isolation and characterization from the sample tubes and the cassettes. Fifty microliters of saliva (three samples/time) were deposited in denim, cotton, and polyester. Saliva was identified by SERATEC® Amylase Test and the Crime Scene version SALIVA CS, being able to detect it up to six months of deposition, although with different band intensities. Polyester showed stronger bands than cotton, probably due to its synthetic nature, and denim, as an inked fabric, showed less band intensities. Statistical analyses confirmed significant differences among fabrics, but not over time in the same type of fabric. Total DNA from the sample tubes was successfully recovered, in contrast, from the cassettes, only polyester retrieved amplifiable DNA. These findings indicated that it is possible to recover and identify saliva up to six months after deposition, also obtaining DNA. Future research will be able to expand these results, analyzing the stability of other body fluids, and the sensitivity of lateral flow immunochromatographic tests to detect them.

Original languageEnglish (US)
Pages (from-to)248-250
Number of pages3
JournalForensic Science International: Genetics Supplement Series
StatePublished - Dec 2022

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Genetics


  • DNA
  • Lateral flow immunochromatographic (LFI) tests
  • Saliva
  • Stability


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