Two-photon absorption and time-resolved stimulated emission depletion spectroscopy of a new fluorenyl derivative

Kevin D. Belfield, Mykhailo V. Bondar, Alma R. Morales, Xiling Yue, Gheorghe Luchita, Olga V. Przhonska, Olexy D. Kachkovsky

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The synthesis, comprehensive linear photophysical characterization, two-photon absorption (2PA), steady-state and time-resolved stimulated emission depletion properties of a new fluorene derivative, (E)-1-(2-(di-p-tolylamino)-9, 9-diethyl-9H-fluoren-7-yl)-3-(thiophen-2-yl)prop-2-en-1-one (1), are reported. The primary linear spectral properties, including excitation anisotropy, fluorescence lifetimes, and photostability, were investigated in a number of aprotic solvents at room temperature. The degenerate 2PA spectra of 1 were obtained with open-aperture Z-scan and two-photon induced fluorescence methods, using a 1 kHz femtosecond laser system, and maximum 2PA cross-sections of ∼400-600 GM were obtained. The nature of the electronic absorption processes in 1 was investigated by DFT-based quantum chemical methods implemented in the Gaussian 09 program. The one- and two-photon stimulated emission spectra of 1 were measured over a broad spectral range using a femtosecond pump-probe-based fluorescence quenching technique, while a new methodology for time-resolved fluorescence emission spectroscopy is proposed. An effective application of 1 in fluorescence bioimaging was demonstrated by means of one- and two-photon fluorescence microscopy images of HCT 116 cells containing dye encapsulated micelles. The one- and two-photon stimulated emission spectra of a new fluorene derivative, (E)-1-(2-(di-p-tolylamino)-9,9-diethyl-9H-fluoren-7-yl)-3-(thiophen- 2-yl)prop-2-en-1-one are obtained over a broad spectral range (see picture) and a new methodology for time-resolved fluorescence emission spectroscopy is proposed.

Original languageEnglish (US)
Pages (from-to)3481-3491
Number of pages11
JournalChemPhysChem
Volume13
Issue number15
DOIs
StatePublished - Oct 22 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry

Keywords

  • bioimaging
  • fluorene derivatives
  • time-resolved fluorescence spectroscopy
  • two-photon absorption
  • two-photon stimulated emission depletion

Fingerprint Dive into the research topics of 'Two-photon absorption and time-resolved stimulated emission depletion spectroscopy of a new fluorenyl derivative'. Together they form a unique fingerprint.

Cite this