Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

Shanshan Wang, Zhiliang Li, Xinglei Liu, Samantha Phan, Fengting Lv, Kevin D. Belfield, Shu Wang, Kirk S. Schanze

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Two cationic conjugated polyelectrolytes (CPEs) based on poly(phenylene ethynylene) are found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates display enhanced 2-photon absorption compared to the nonaggregated states. The CPEs are used as 2-photon absorption sensitizers to harvest light and amplify the fluorescence from green fluorescent protein (GFP) through Förster resonance energy transfer. Considerably enhanced GFP fluorescence under near-infrared 2-photon excitation is demonstrated both in solution and in HeLa cells. The results suggest applications in 2-photon fluorescence imaging of cells, tissues, and organs of living animals expressing GFP.

Original languageEnglish (US)
Pages (from-to)3295-3303
Number of pages9
JournalChemistry of Materials
Volume29
Issue number7
DOIs
StatePublished - Apr 11 2017

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

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