Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

Shanshan Wang; Zhiliang Li; Xinglei Liu; Samantha Phan; Fengting Lv; Kevin D. Belfield; Shu Wang; Kirk S. Schanze

doi:10.1021/acs.chemmater.7b00676

Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

Shanshan Wang, Zhiliang Li, Xinglei Liu, Samantha Phan, Fengting Lv, Kevin D. Belfield, Shu Wang, Kirk S. Schanze

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Two cationic conjugated polyelectrolytes (CPEs) based on poly(phenylene ethynylene) are found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates display enhanced 2-photon absorption compared to the nonaggregated states. The CPEs are used as 2-photon absorption sensitizers to harvest light and amplify the fluorescence from green fluorescent protein (GFP) through Förster resonance energy transfer. Considerably enhanced GFP fluorescence under near-infrared 2-photon excitation is demonstrated both in solution and in HeLa cells. The results suggest applications in 2-photon fluorescence imaging of cells, tissues, and organs of living animals expressing GFP.

Original language	English (US)
Pages (from-to)	3295-3303
Number of pages	9
Journal	Chemistry of Materials
Volume	29
Issue number	7
DOIs	https://doi.org/10.1021/acs.chemmater.7b00676
State	Published - Apr 11 2017

All Science Journal Classification (ASJC) codes

General Chemistry
General Chemical Engineering
Materials Chemistry

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title = "Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein",

abstract = "Two cationic conjugated polyelectrolytes (CPEs) based on poly(phenylene ethynylene) are found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates display enhanced 2-photon absorption compared to the nonaggregated states. The CPEs are used as 2-photon absorption sensitizers to harvest light and amplify the fluorescence from green fluorescent protein (GFP) through F{\"o}rster resonance energy transfer. Considerably enhanced GFP fluorescence under near-infrared 2-photon excitation is demonstrated both in solution and in HeLa cells. The results suggest applications in 2-photon fluorescence imaging of cells, tissues, and organs of living animals expressing GFP.",

author = "Shanshan Wang and Zhiliang Li and Xinglei Liu and Samantha Phan and Fengting Lv and Belfield, \{Kevin D.\} and Shu Wang and Schanze, \{Kirk S.\}",

note = "Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

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doi = "10.1021/acs.chemmater.7b00676",

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T1 - Two-Photon Absorption of Cationic Conjugated Polyelectrolytes

T2 - Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

AU - Wang, Shanshan

AU - Li, Zhiliang

AU - Liu, Xinglei

AU - Phan, Samantha

AU - Lv, Fengting

AU - Belfield, Kevin D.

AU - Wang, Shu

AU - Schanze, Kirk S.

PY - 2017/4/11

Y1 - 2017/4/11

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AB - Two cationic conjugated polyelectrolytes (CPEs) based on poly(phenylene ethynylene) are found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates display enhanced 2-photon absorption compared to the nonaggregated states. The CPEs are used as 2-photon absorption sensitizers to harvest light and amplify the fluorescence from green fluorescent protein (GFP) through Förster resonance energy transfer. Considerably enhanced GFP fluorescence under near-infrared 2-photon excitation is demonstrated both in solution and in HeLa cells. The results suggest applications in 2-photon fluorescence imaging of cells, tissues, and organs of living animals expressing GFP.

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Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein

Abstract

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