Abstract
The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO3, was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO3-coated silica nanoparticles in HeLa cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.
Original language | English (US) |
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Pages (from-to) | 2744-2748 |
Number of pages | 5 |
Journal | ACS Applied Materials and Interfaces |
Volume | 2 |
Issue number | 10 |
DOIs | |
State | Published - Oct 27 2010 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- General Materials Science
Keywords
- cell imaging
- conjugated polyelectrolytes
- near-infrared
- two-photon absorption