Two-photon excited fluorescence of a conjugated polyelectrolyte and its application in cell imaging

Anand Parthasarathy, Hyo Yang Ahn, Kevin D. Belfield, Kirk S. Schanze

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO3, was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO3-coated silica nanoparticles in HeLa cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.

Original languageEnglish (US)
Pages (from-to)2744-2748
Number of pages5
JournalACS Applied Materials and Interfaces
Volume2
Issue number10
DOIs
StatePublished - Oct 27 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Keywords

  • cell imaging
  • conjugated polyelectrolytes
  • near-infrared
  • two-photon absorption

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