Two-photon excited fluorescence of a conjugated polyelectrolyte and its application in cell imaging

Anand Parthasarathy; Hyo Yang Ahn; Kevin D. Belfield; Kirk S. Schanze

doi:10.1021/am100784m

Two-photon excited fluorescence of a conjugated polyelectrolyte and its application in cell imaging

Anand Parthasarathy
, Hyo Yang Ahn
, Kevin D. Belfield
, Kirk S. Schanze

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO₃, was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO₃-coated silica nanoparticles in HeLa cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.

Original language	English (US)
Pages (from-to)	2744-2748
Number of pages	5
Journal	ACS Applied Materials and Interfaces
Volume	2
Issue number	10
DOIs	https://doi.org/10.1021/am100784m
State	Published - Oct 27 2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Materials Science

Keywords

cell imaging
conjugated polyelectrolytes
near-infrared
two-photon absorption

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10.1021/am100784m

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title = "Two-photon excited fluorescence of a conjugated polyelectrolyte and its application in cell imaging",

abstract = "The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO3, was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO3-coated silica nanoparticles in HeLa cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.",

keywords = "cell imaging, conjugated polyelectrolytes, near-infrared, two-photon absorption",

author = "Anand Parthasarathy and Ahn, \{Hyo Yang\} and Belfield, \{Kevin D.\} and Schanze, \{Kirk S.\}",

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AU - Parthasarathy, Anand

AU - Ahn, Hyo Yang

AU - Belfield, Kevin D.

AU - Schanze, Kirk S.

PY - 2010/10/27

Y1 - 2010/10/27

N2 - The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO3, was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO3-coated silica nanoparticles in HeLa cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.

AB - The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO3, was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO3-coated silica nanoparticles in HeLa cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.

KW - cell imaging

KW - conjugated polyelectrolytes

KW - near-infrared

KW - two-photon absorption

UR - https://www.scopus.com/pages/publications/79151471288

UR - https://www.scopus.com/pages/publications/79151471288#tab=citedBy

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SN - 1944-8244

VL - 2

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EP - 2748

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 10

ER -

Two-photon excited fluorescence of a conjugated polyelectrolyte and its application in cell imaging

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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