Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe

Carolina D. Andrade; Ciceron O. Yanez; Maher A. Qaddoura; Xuhua Wang; Curtesa L. Arnett; Sabrina A. Coombs; Jin Yu; Rania Bassiouni; Mykhailo V. Bondar; Kevin D. Belfield

doi:10.1007/s10895-010-0801-3

Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe

Carolina D. Andrade, Ciceron O. Yanez, Maher A. Qaddoura, Xuhua Wang, Curtesa L. Arnett, Sabrina A. Coombs, Jin Yu, Rania Bassiouni, Mykhailo V. Bondar, Kevin D. Belfield

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

Original language	English (US)
Pages (from-to)	1223-1230
Number of pages	8
Journal	Journal of Fluorescence
Volume	21
Issue number	3
DOIs	https://doi.org/10.1007/s10895-010-0801-3
State	Published - May 2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Clinical Psychology
Social Sciences (miscellaneous)
Sociology and Political Science
Spectroscopy
Clinical Biochemistry
Law

Keywords

Bioimaging
Fluorescence microscopy
Fluorescent dyes
Lysosomes
Multiphoton absorption
Pluronic

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10.1007/s10895-010-0801-3

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title = "Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe",

abstract = "We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic{\textregistered} F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.",

keywords = "Bioimaging, Fluorescence microscopy, Fluorescent dyes, Lysosomes, Multiphoton absorption, Pluronic",

author = "Andrade, {Carolina D.} and Yanez, {Ciceron O.} and Qaddoura, {Maher A.} and Xuhua Wang and Arnett, {Curtesa L.} and Coombs, {Sabrina A.} and Jin Yu and Rania Bassiouni and Bondar, {Mykhailo V.} and Belfield, {Kevin D.}",

note = "Funding Information: Acknowledgments This work was supported by the National Institutes of Health (1 R15 EB008858-01), the National Science Foundation (CHE-0832622 and CHE-0840431), the US Civilian Research and Development Foundation (UKB2-2923-KV-07), and the Ministry of Education and Science of Ukraine (grant M/49-2008).",

year = "2011",

month = may,

doi = "10.1007/s10895-010-0801-3",

language = "English (US)",

volume = "21",

pages = "1223--1230",

journal = "Journal of Fluorescence",

issn = "1053-0509",

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T1 - Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe

AU - Andrade, Carolina D.

AU - Yanez, Ciceron O.

AU - Qaddoura, Maher A.

AU - Wang, Xuhua

AU - Arnett, Curtesa L.

AU - Coombs, Sabrina A.

AU - Yu, Jin

AU - Bassiouni, Rania

AU - Bondar, Mykhailo V.

AU - Belfield, Kevin D.

N1 - Funding Information: Acknowledgments This work was supported by the National Institutes of Health (1 R15 EB008858-01), the National Science Foundation (CHE-0832622 and CHE-0840431), the US Civilian Research and Development Foundation (UKB2-2923-KV-07), and the Ministry of Education and Science of Ukraine (grant M/49-2008).

PY - 2011/5

Y1 - 2011/5

N2 - We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

AB - We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

KW - Bioimaging

KW - Fluorescence microscopy

KW - Fluorescent dyes

KW - Lysosomes

KW - Multiphoton absorption

KW - Pluronic

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SN - 1053-0509

VL - 21

SP - 1223

EP - 1230

JO - Journal of Fluorescence

JF - Journal of Fluorescence

IS - 3

ER -

Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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