Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe

Carolina D. Andrade, Ciceron O. Yanez, Maher A. Qaddoura, Xuhua Wang, Curtesa L. Arnett, Sabrina A. Coombs, Jin Yu, Rania Bassiouni, Mykhailo V. Bondar, Kevin D. Belfield

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

Original languageEnglish (US)
Pages (from-to)1223-1230
Number of pages8
JournalJournal of Fluorescence
Issue number3
StatePublished - May 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Psychology
  • Social Sciences (miscellaneous)
  • Sociology and Political Science
  • Spectroscopy
  • Clinical Biochemistry
  • Law


  • Bioimaging
  • Fluorescence microscopy
  • Fluorescent dyes
  • Lysosomes
  • Multiphoton absorption
  • Pluronic


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