Two-photon STED spectral determination for a new V-shaped organic fluorescent probe with efficient two-photon absorption

Kevin D. Belfield, Mykhailo V. Bondar, Alma R. Morales, Lazaro A. Padilha, Olga V. Przhonska, Xuhua Wang

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Two-photon stimulated emission depletion (STED) cross sections were determined over a broad spectral range for a novel two-photon absorbing organic molecule, representing the first such report. The synthesis, comprehensive linear photophysical, two-photon absorption (2PA), and stimulated emission properties of a new fluorene-based compound, (E)-2-{3-[2-(7-(diphenylamino)-9,9- diethyl-9H-fluoren-2-yl)vinyl]-5-methyl-4-oxocyclohexa-2,5-dienylidene} malononitrile (1), are presented. Linear spectral parameters, including excitation anisotropy and fluorescence lifetimes, were obtained over a broad range of organic solvents at room temperature. The degenerate two-photon absorption (2PA) spectrum of 1 was determined with a combination of the direct open-aperture Z-scan and relative two-photon-induced fluorescence methods using 1 kHz femtosecond excitation. The maximum value of the 2PA cross section ∼1700 GM was observed in the main, long wavelength, one-photon absorption band. One- and two-photon stimulated emission spectra of 1 were obtained over a broad spectral range using a femtosecond pump-probe technique, resulting in relatively high two-photon stimulated emission depletion cross sections (∼1200 GM). A potential application of 1 in bioimaging was demonstrated through one- and two-photon fluorescence microscopy images of HCT 116 cells incubated with micelle-encapsulated dye. One, two, let's go! One- and two-photon stimulated emission spectra of 1 (left) are obtained over a broad spectral region by a fluorescence quenching femtosecond pump-probe technique (right).

Original languageEnglish (US)
Pages (from-to)2755-2762
Number of pages8
JournalChemPhysChem
Volume12
Issue number15
DOIs
StatePublished - Oct 24 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry

Keywords

  • absorption
  • fluorene derivatives
  • fluorescence
  • laser scanning fluorescence microscopy
  • two-photon stimulated emission depletion

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