Zn sorption mechanisms onto sheathed Leptothrix discophora and the impact of the nanoparticulate biogenic Mn oxide coating

Thipnakarin Boonfueng, Lisa Axe, Nathan Yee, Dittmar Hahn, Peter K. Ndiba

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Zinc sorption on sheathed Leptothrix discophora bacterium, the isolated extracellular polymeric substances (EPS) sheath, and Mn oxide-coated bacteria was investigated with macroscopic and spectroscopic techniques. Complexation with L. discophora was dominated by the outer membrane phosphoryl groups of the phospholipid bilayer while sorption to isolated EPS was dominated by carboxyl groups. Precipitation of nanoparticulate Mn oxide coatings on the cell surface increased site capacity by over twenty times with significant increase in metal sorption. XAS analysis of Zn sorption in the coated system showed Mn oxide phase contributions of 18 to 43% through mononuclear inner-sphere complexes. The coordination environments in coprecipitation samples were identical to those of sorption samples, indicating that, even in coprecipitation, Zn is not incorporated into the Mn oxide structure. Rather, through enzymatic oxidation by L. discophora, Mn(II) is oxidized and precipitated onto the biofilm providing a large surface for metal sequestration. The nanoparticulate Mn oxide coating exhibited significant microporosity (75%) suggesting contributions from intraparticle diffusion. Transient studies conducted over 7 months revealed a 170% increase in Zn loading. However, the intraparticle diffusivity of 10-19 cm2 s-1 is two orders of magnitude smaller than that for abiotic Mn oxide which we attribute to morphological changes such as reduced pore sizes in the nanoparticulate oxide. Our results demonstrate that the cell-bound Mn oxide particles can sorb significant amounts of Zn over long periods of time representing an important surface for sequestration of metal contaminants.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalJournal of Colloid And Interface Science
Volume333
Issue number2
DOIs
StatePublished - May 15 2009

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

Keywords

  • Biogenic manganese oxide
  • Diffusion
  • Extracellular polymeric substances
  • Intraparticle
  • Leptothrix discophora
  • Sheath
  • XAS
  • Zn
  • coprecipitation

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